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001			234494
003			ES-VaUE
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007			cr nn 008mamaa
008			161207s2017 gw | s |||| 0|eng d
020			_a9783662531150
024	7		_a10.1007/978-3-662-53115-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHydrocarbon and Lipid Microbiology Protocols _bBioproducts, Biofuels, Biocatalysts and Facilitating Tools _cedited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales.
250			_a1st edition 2017
264		1	_aBerlin, Heidelberg _bSpringer International Publising _c2017
300			_a1 recurso en línea (X, 245 páginas) _b50 ilustraciones, 30 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aIntroduction -- Genetic enzyme screening system: a method for high-throughput functional screening of novel enzymes from metagenome libraries -- Functional screening of metagenomic libraries: enzymes acting on greasy molecules as study case -- Screening for Enantioselective Lipases -- Use of bacterial polyhydroxyalkanoates in protein display technologies -- Bacterial Secretion Systems for Use in Biotechnology:Autotransporter-Based Cell-Surface Display and Ultra-High Throughput Screening of Large Protein Libraries -- Protocols for the isolation and preliminary characterization of bacteria for biodesulfurization and biodenitrogenation of petroleum-derived fuels -- Syngas fermentation for polyhydroxyalkanoate production in Rhodospirillum rubrum -- Genetic strategies on Kennedy pathway to improve triacylglycerol production in oleaginous Rhodococcus strains -- Production of biofuel-related isoprenoids derived from Botryococcus braunii algae -- Protocols for monitoring growth and lipid accumulation in oleaginous yeasts -- Protocol for start-up and operation of CSTR biogas processes -- Protocols for the use of gut models to study the potential contribution of the gut microbiota to human nutrition through the production of short chain fatty acids -- Protocol for the application of bioluminescence full-cell bioreporters for monitoring of terrestrial bioremediation.
520			_aThis Volume covers protocols for various applications in hydrocarbon microbiology, including those of interest for industrial processes, biocatalysis, lipid and biofuel production, bioproducts, or the human microbiome. It presents detailed protocols for the functional screening of enzymes acting on greasy molecules (i.e. lipases, esterases), including assays for enantioselective biocatalysts, as well as approaches for protein display technologies. Protocols for improving fuel quality and production of biofuel and lipids in different hosts (bacteria, algae, yeast) are also provided. The production of biogas from organic waste and its fermentation into value-added products such as polyhydroxyalkanoates is covered, as well as an in-vitro model of the gut microbiome for short-chain fatty acid metabolism and microbial diversity analyses. The applications presented are examples of the many potential applications in hydrocarbon and lipid microbiology, and many (i.e. protein-display technologies) will also be of interest in other research fields. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
700	1		_aMcGenity, Terry J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aTimmis, Kenneth N _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aNogales, Balbina _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9783662531136
776	0	8	_iPrinted edition: _z9783662531143
776	0	8	_iPrinted edition: _z9783662571149
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-662-53115-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234494 _d234494