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001			234498
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007			cr nn 008mamaa
008			161021s2017 xxu| s |||| 0|eng d
020			_a9781489977083
024	7		_a10.1007/978-1-4899-7708-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Epigenetics _bMethods and Protocols _cedited by Igor Kovalchuk.
250			_a2nd edition 2017
264		1	_aNew York, NY _bSpringer International Publising _c2017
300			_a1 recurso en línea (X, 253 páginas) _b51 ilustraciones, 32 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1456
505	0		_aChromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis -- Chromatin Conformation Capture-Based Analysis of Nuclear Architecture -- Metaanalysis of Genome-Wide Chromatin Data -- Localization of Mirnas by In Situ Hybridization in Plants using Conventional Oligonucleotide Probes -- COBRA (Combined Bisulfite Restriction Analysis) Assay for the Analysis of Locus-Specific Changes in Methylation Patterns -- Analysis of Global Genome Methylation Using the Cytosine-Extension Assay -- In Situ Analysis Of DNA Methylation In Plants -- Analysis of DNA Hydroxymethylation In Arabidopsis Using Colorimetric Assay -- Analysis of DNA Cytosine Methylation Patterns using Methylation Specific Amplification Polymorphism (MSAP) -- Differentially Methylated Region - Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify Dmrs in Uncharacterized Genomes -- Analysis of Small RNA Populations Using Hybridization to DNA Tiling Arrays -- Northern Blotting Techniques for Small RNAs -- Stem-Loop Qrt-PCR for the Detection of Plant Micrornas -- Profiling New Small RNA Sequences -- Small RNA Library Preparation and Illumina Sequencing in Plants -- Bioinformatics Analysis of Small RNA Transcriptomes - The Detailed Work Flow -- Increasing a Stable Transformation Efficiency of Arabidopsis by Manipulating the Endogenous Gene Expression using Virus-Induced Gene Silencing -- The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNA Strand Breaks -- Profiling Transposable Elements and their Epigenetic Effects in Non-Model Species.
520			_aThis volume provides a variety of protocols to analyze various epigenetic changes, including differential expression of non-coding RNAs, changes in DNA methylation, and histone modifications in plants. Chapters detail protocols with different degrees of complexity, and describe bioinformatics approaches for data processing and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Epigenetics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field. .
700	1		_aKovalchuk, Igor _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489977069
776	0	8	_iPrinted edition: _z9781489977076
776	0	8	_iPrinted edition: _z9781493979561
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4899-7708-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234498 _d234498