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008			100301s2002 xxu| s |||| 0|eng d
020			_a9781592592906
024	7		_a10.1385/1592592902 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPeptide Nucleic Acids _bMethods and Protocols _cedited by Peter E. Nielsen.
250			_a1st edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (X, 274 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v208
505	0		_aPNA Technology -- Chemistry -- Solid Phase Synthesis of PNA Oligomers -- Synthesis of PNA-Peptide Conjugates -- Parallel Synthesis of PNA-Peptide Conjugate Libraries -- Thermodynamics of PNA Interactions with DNA and RNA -- PNA Technology -- Measurement of PNA Binding to Double-Stranded DNA -- PNA-Mediated Immobilization of Supercoiled DNA -- PNA Openers and Their Applications -- Colorimetric Detection of PNA-DNA Hybridization Using Cyanine Dyes -- Diagnostics -- PNA-Mediated PCR Clamping -- PNA Clamping Techniques for the Determination of Oncogene Mutations -- PNA Fluorescent In Situ Hybridization for Rapid Microbiology and Cytogenetic Analysis -- Detection of Point Mutations Using PNA-Containing Electrophoresis Matrices -- Antisense and Antigene -- Lipid-Mediated Introduction of Peptide Nucleic Acids Into Cells -- Synthesis of Cell-Penetrating Peptide-PNA Constructs -- Antisense Inhibition of Bacterial Gene Expression and Cell Growth -- In Vitro Transcription from Peptide Nucleic Acid/DNA Strand Displacement Loops -- Animal Studies -- In Vitro and In Vivo Studies on the Pharmacokinetics and Metabolism of PNA Constructs in Rodents.
520			_aPeptide nucleic acid (PNA) technology continues to grow in importance throughout molecular biology, genetic diagnostics, and molecular medicine. In Peptide Nucleic Acids: Methods and Protocols, Peter Eigil Nielsen has assembled a critically evaluated collection of PNA protocols that are either already well established around the world, such as PCR-clamping and in situ hybridization, or that display promise of significant future impact. Basic methods for PNA oligomer synthesis and analysis have also been included. Written by experts with hands-on experience in the methods they describe, these readily reproducible protocols contain detailed step-by-step instructions, tips on avoiding pitfalls and on extending the method to other situations, and introductory material explaining the theory behind the process. Up-to-date and highly practical, Peptide Nucleic Acids: Methods and Protocols provides for both novice and experienced researchers a thorough and easy introduction to the optimal use of today's PNA technology across a wide variety of fields, including molecular biology, medicinal chemistry, genetic diagnostics, and drug development.
700	1		_aNielsen, Peter E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617372858
776	0	8	_iPrinted edition: _z9780896039766
776	0	8	_iPrinted edition: _z9781489938664
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592902 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2002
999			_c234503 _d234503