000			04294nam a22003615i 4500
001			234504
003			ES-VaUE
005			20221220020606.0
007			cr nn 008mamaa
008			130531s2013 xxu| s |||| 0|eng d
020			_a9781627034531
024	7		_a10.1007/978-1-62703-453-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCirculating MicroRNAs _bMethods and Protocols _cedited by Nobuyoshi Kosaka.
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XI, 270 páginas) _b44 ilustraciones, 22 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1024
505	0		_aFunctional Analysis of Exosomal MicroRNA in Cell-Cell Communication Research -- Isolation of Extracellular Nanovesicle MicroRNA from Liver Cancer Cells in Culture -- Methods of Analysis of Dendritic Cell-derived Exosome-shuttle MicroRNA and its Horizontal Propagation Between Dendritic Cells -- Analysis of MicroRNA and Protein Transfer by Exosomes During an Immune Synapse -- Analysis of Viral MicroRNA Exchange via Exosomes In Vitro and In Vivo -- Measurement of Precursor miRNA in Exosomes from Human ESC-Derived Mesenchymal Stem Cells -- Analysis of the Transfer of Circulating microRNA Between Cells Mediated by Gap Junction -- Isolation of Circulating MicroRNA Associated with RNA-binding Protein -- Identification and Analysis of Circulating Exosomal microRNA in Human Body Fluids -- Analyzing the Circulating MicroRNAs in Exosomes/Extracellular Vesicles from Serum or Plasma by qRT-PCR -- Direct Serum Assay for MicroRNA in Cancer Patients -- Analysis of MicroRNA Niches: Techniques to Measure Extracellular MicroRNA and Intracellular MicroRNA In Situ -- Analysis of Circulating MicroRNA by Microarray in Liver Disease -- Isolation of Circulating MicroRNA in Saliva -- Purification of RNA From Milk Whey -- Circulating MicroRNAs in the Cerebrospinal Fluid of Patients with Brain Diseases -- Methods of MicroRNA Quantification in Urinary Sediment -- Circulating microRNA for the Identification of Forensically Relevant Body Fluids -- Identification of Prostate Cancer-associated MicroRNAs in Circulation using a Mouse Model of Disease -- A Combination of Extraction Reagent and DNA Microarray that Allows for the Detection of Global MiRNA Profiles from Serum /Plasma -- Nanopore Single-molecule Detection of Circulating microRNAs.
520			_aMicroRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In Circulating MicroRNAs: Methods and Protocols, expert researchers in the field detail recent advances in the isolation, purification and analysis of circulating miRNAs from a variety of sources for research. The book is divided into three main topics. The first section involves the study of secretory miRNAs in cell-cell communication, and the second, the study of circulating miRNAs in body fluids. The last describes the novel techniques used to study circulating miRNAs. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Circulating MicroRNAs: Methods and Protocols seeks to aid scientists in dealing with the recent advances of RNAi technology from the bench to the bedside.
700	1		_aKosaka, Nobuyoshi _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627034548
776	0	8	_iPrinted edition: _z9781627034524
776	0	8	_iPrinted edition: _z9781493962990
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-453-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c234504 _d234504