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001			234505
003			ES-VaUE
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007			cr nn 008mamaa
008			161226s2017 gw | s |||| 0|eng d
020			_a9783662504505
024	7		_a10.1007/978-3-662-50450-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHydrocarbon and Lipid Microbiology Protocols _bGenetic, Genomic and System Analyses of Communities _cedited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales.
250			_a1st edition 2017
264		1	_aBerlin, Heidelberg _bSpringer International Publising _c2017
300			_a1 recurso en línea (X, 288 páginas) _b50 ilustraciones, 30 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aIntroduction to genetic, genomic and system analyses for communities -- Genomic analysis of pure cultures and communities -- Protocols for metagenomic library generation and analysis in petroleum hydrocarbon microbe systems -- Preparation and analysis of metatranscriptomic libraries in petroleum hydrocarbon microbe systems -- Prokaryotic metatranscriptomics -- Defining a pipeline for metaproteomic analyses -- Generating enriched metagenomes from active microorganisms with DNA stable isotope probing -- DNA and RNA-based stable isotope probing of hydrocarbon degraders -- Protein stable isotope probing (Protein-SIP) -- Protein extraction from contaminated soils and sediments -- Analysis of the regulation of the rate of hydrocarbon and nutrient flow through microbial communities -- Physiological modelling of complex bacterial communities -- Protocol for evaluating the permissiveness of bacterial communities towards conjugal plasmids by quantification and isolation of transconjugants.
520			_aThis Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities. Methods are described for the creation and bioinformatic assessment of metagenomic and metatranscriptomic libraries, and for metaproteomic analyses, which provide important insights into the metabolic potential and interactions of community members. These in turn lead to specific hypotheses concerning the functional contributions of individual populations in the community, which may be investigated by the stable isotope probing approaches described in this Volume, making it possible to identify those community members primarily responsible for particular functions. Methods for the direct extraction of proteins from environmental samples for sequencing and activity tests are presented, providing a broad overview of prevailing metabolic activities and of the types of microbe involved in them. Protocols for the analysis of nutrient flow through microbial communities and for the modelling of dynamic physiological interactions in communities are also provided. Lastly, the book presents a protocol for the quantitative assessment of permissiveness for the transfer of conjugative plasmids, important agents of physiological change and evolution in microbial communities. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
700	1		_aMcGenity, Terry J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aTimmis, Kenneth N _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aNogales, Balbina _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9783662504499
776	0	8	_iPrinted edition: _z9783662504512
776	0	8	_iPrinted edition: _z9783662570623
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-662-50450-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234505 _d234505