000 04169nam a22003735i 4500
001 234508
003 ES-VaUE
005 20221220020606.0
007 cr nn 008mamaa
008 100301s1995 xxu| s |||| 0|eng d
020 _a9781592595310
024 7 _a10.1385/0896033023
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aAgrobacterium Protocols
_cedited by Kevan M. A. Gartland, Michael R. Davey.
250 _a1st edition 1995
264 1 _aTotowa, NJ
_bHumana Press
_c1995
300 _a1 recurso en línea (XIV, 417 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v44
505 0 _aGrowth and Storage of Agrobacterium -- Simple Cultural Tests for Identification of Agrobacterium Biovars -- Agrobacterium Virulence -- Agrobacterium tumefaciens Chemotaxis Protocols -- Effect of Acetosyringone on Growth and Oncogenic Potential of Agrobacterium tumefaciens -- Binary Ti Plasmid Vectors -- Leaf Disk Transformation -- Agrobacterium-Mediated Transformation of Protoplasts from Oilseed Rape (Brassica napus L.) -- Agrobacterium-Mediated Transformation of Stem Disks from Oilseed Rape (Brassica napus L.) -- Peanut Transformation -- Transformation of Soybean (Glycine max) via Agrobacterium tumefaciens and Analysis of Transformed Plants -- Agrobacterium-Mediated Transformation of Potato Genotypes -- Agrobacterium-Mediated Transformation of Soft Fruit Rubus, Ribes, and Fragaria -- High-Frequency and Efficient Agrobacterium-Mediated Transformation ofArabidopsis thaliana Ecotypes "C24" and "Landsberg erecta" Using Agrobacterium tumefaciens -- Transformation Protocols for Broadleaved Trees -- Agrobacterium-Mediated Antibiotic Resistance for Selection of Somatic Hybrids -- Histochemical GUS Analysis -- Fluorometric GUS Analysis for Transformed Plant Material -- The Detection of Neomycin Phosphotransferase Activity in Plant Extracts -- The Plant Oncogenes rolA, B, and C from Agrobacterium rhizogenes -- Quantifying Polyamines in Agrobacterium rhizogenes Strains and in Ri Plasmid Transformed Cells -- IAA Analysis in Transgenic Plants -- Quantifying Phytohormones in Transformed Plants -- Manipulating Photosynthesis in Transgenic Plants -- Gene Activation by T-DNA Tagging -- Assessing Cadmium Partitioning in Transgenic Plants -- Overexpression of Chloroplastic Cu/Zn Superoxide Dismutase in Plants -- Agroinfection -- T-DNA Transfer to Maize Plants -- Use of Cosmid Libraries in Plant Transformations -- Regulation of Agrobacterium Gene Manipulation -- Transgenic Oilseed Rape -- Electroporation Protocols for Agrobacterium.
520 _aAgrobacterium Protocols offers beginning and experienced researchers the most comprehensive collection of step-by-step protocols for the genetic manipulation of plants using Agrobacterium. The topics range from the maintenance of bacterial culture collections to aspects of the metabolism and physiology of transformed tissues and transgenic plants. Drawing on the work of leading scientists from laboratories around the world, Agrobacterium Protocols provides a wealth of techniques for introducing specific DNA sequences into target plant species and discusses the environmental implications of genetically engineered plants. Its detailed procedures will facilitate rapid transfer of advanced techniques to other laboratories and their exploitation in fundamental and applied plant biology.
700 1 _aGartland, Kevan M. A
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aDavey, Michael R
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781489927996
776 0 8 _iPrinted edition:
_z9781489927989
776 0 8 _iPrinted edition:
_z9780896033023
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033023
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_1995
999 _c234508
_d234508