000 04490nam a22003615i 4500
001 234516
003 ES-VaUE
005 20221220020607.0
007 cr nn 008mamaa
008 101109s2011 xxu| s |||| 0|eng d
020 _a9781607619628
024 7 _a10.1007/978-1-60761-962-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aEmbryonic Stem Cell Therapy for Osteo-Degenerative Diseases
_bMethods and Protocols
_cedited by Nicole I. Nieden.
250 _a1st edition 2011
264 1 _aTotowa, NJ
_bHumana Press
_c2011
300 _a1 recurso en línea (XII, 336 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v690
505 0 _aEmbryonic Stem Cells for Osteo-Degenerative Diseases -- Methods for Culturing Mouse and Human Embryonic Stem Cells -- Serum-Free and Feeder free Culture Conditions for Human Embryonic Stem Cells -- Functional Assays for Human Embryonic Stem Cell Pluripotency -- Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem Cells -- Generation of Human Embryonic Stem Cells Carrying Lineage Specific Reporters -- Manipulations of MicroRNA in Human Pluripotent Stem Cells and Their Derivatives -- Large-Scale Expansion of Mouse Embryonic Stem Cells on Microcarriers -- Embryoid Body Formation: Recent Advances in Automated Bioreactor Technology -- Methods for Embryoid Body Formation: The Microwell Approach -- Human Embryonic Stem Cell-Derived Mesenchymal Progenitors: An Overview -- Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells -- Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the "Raclure" Method -- Improved Media Compositions for the Differentiation of Embryonic Stem Cells into Osteoblasts and Chondrocytes -- Differentiation of Mouse Embryonic Stem Cells in Self-Assembling Peptide Scaffolds -- Methods for Investigation of Osteoclastogenesis Using Mouse Embryonic Stem Cells -- Absorption-Based Assays for the Analysis of Osteogenic and Chondrogenic Yield -- Identification of Osteoclasts in Culture -- Analysis of Glycosaminoglycans in Stem Cell Glycomics -- Drill Hole Defects: Induction, Imaging, and Analysis in the Rodent -- Measurement and Illustration of Immune Interaction after Stem Cell Transplantation.
520 _aEmbryonic stem cells (ESCs) offer an unlimited self-renewing capacity, as opposed to the limits of adult stem cells; therefore, ESCs represent an almost bottomless resource for regenerative medicine and tissue engineering approaches. In Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols, accomplished investigators provide detailed descriptions on how to expand ESCs from the most commonly used species ex vivo, i.e. mouse and human, in static culture as well as in controllable bioreactor processes. The thorough and timely volume summarizes the methods that may be used to differentiate these cells along the desired lineage of choice, be it osteoblasts, osteoclasts, or chondrocytes, and consequentially also offers analysis tools for the characterization of resulting cells and evaluation of differentiation effectiveness. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Dependable and cutting-edge, Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols supplies the tools necessary to allow researchers to carry out critical research needed in order to bring this burgeoning and vitally important field closer to the clinic and to ensure the widespread application of a successful strategy.
700 1 _aNieden, Nicole I
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781607619611
776 0 8 _iPrinted edition:
_z9781607619635
776 0 8 _iPrinted edition:
_z9781493957231
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-962-8
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2011
999 _c234516
_d234516