000			03348nam a22003615i 4500
001			234518
003			ES-VaUE
005			20221220020607.0
007			cr nn 008mamaa
008			100301s2008 xxu| s |||| 0|eng d
020			_a9781597452328
024	7		_a10.1007/978-1-59745-232-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aChromosomal Mutagenesis _cedited by Greg Davis, Kevin J. Kayser.
250			_a1st edition 2008
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XVI, 236 páginas) _b39 ilustraciones, 3 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v435
505	0		_aBiallelic Gene Knockouts in Chinese Hamster Ovary Cells -- Highly Proficient Gene Targeting by Homologous Recombination in the Human Pre-B Cell Line Nalm-6 -- Robust Cell Line Development Using Meganucleases -- Design and Testing of Zinc Finger Nucleases for Use in Mammalian Cells -- Gene Targeting in Drosophila and Caenorhabditis elegans With Zinc-Finger Nucleases -- Orpheus Recombination -- Transposon-Mediated Mutagenesis in Somatic Cells -- Insertional Mutagenesis of the Mouse Germline With Sleeping Beauty Transposition -- Conditional Gene Trapping Using the FLEx System -- Steps Toward Targeted Insertional Mutagenesis With Class II Transposable Elements -- Targeting Integration of the Saccharomyces Ty5 Retrotransposon -- Site-Specific Chromosomal Integration Mediated by ?C31 Integrase -- Triplex-Mediated Gene Modification -- Allelic Exchange of Unmarked Mutations in Mycobacterium Tuberculosis -- Mycobacterial Recombineering -- Chromosomal Engineering of Clostridium Perfringens Using Group II Introns.
520			_aGreat disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation. In Chromosomal Mutagenesis, a team of experts provide a variety of chromosomal manipulation techniques, including insertional gene disruptions, gene knockouts, stimulated homologous recombination techniques and other novel tools, for both prokaryotic and eukaryotic organisms, and attempt to expand the genetic toolbox beyond model organisms. Following the format of the highly successful Methods in Molecular Biology™ format, each chapter offers step-by-step laboratory instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Chomosomal Mutagenesis covers state-of-the-art techniques that are staged to expand, if not revolutionize, genetic analysis in the long neglected and relevant cell types.
700	1		_aDavis, Greg _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aKayser, Kevin J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617378331
776	0	8	_iPrinted edition: _z9781588298997
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-232-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2008
999			_c234518 _d234518