000			03242nam a22003495i 4500
001			234519
003			ES-VaUE
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007			cr nn 008mamaa
008			100301s2007 xxu| s |||| 0|eng d
020			_a9781597454308
024	7		_a10.1007/978-1-59745-430-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPeptide Characterization and Application Protocols _cedited by Gregg B. Fields.
250			_a1st edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XII, 342 páginas) _b102 ilustraciones, 4 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v386
505	0		_aCharacterization -- HPLC Analysis and Purification of Peptides -- Identification of Proteins Based on MS/MS Spectra and Location of Posttranslational Modifications -- Determination of Phosphorylated and O-Glycosylated Sites by Chemical Targeting (CTID) at Ambient Temperature -- Synthesis, Biosynthesis, and Characterization of Transmembrane Domains of a G Protein-Coupled Receptor -- Applications -- Application of Topologically Constrained Mini-Proteins as Ligands, Substrates, and Inhibitors -- Proteolytic Profiling of the Extracellular Matrix Degradome -- ?-Amyloid Protein Aggregation -- Radiometal-Labeled Somatostatin Analogs for Applications in Cancer Imaging and Therapy -- Cell-Penetrating Proline-Rich Peptidomimetics -- Peptide-Mediated Targeting of Liposomes to Tumor Cells -- Peptide-Mediated Delivery of Nucleic Acids into Mammalian Cells -- Broth Microdilution Antibacterial Assay of Peptides -- Depsipeptide Synthesis.
520			_aPeptide Characterization and Application Protocols is dedicated entirely to the characterization of peptides and their applications for the study of biochemical systems and the contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques, including high-performance liquid chromatography (HPLC) for purification and evaluation of peptides and mass spectrometry (MS) for the analysis of standard and modified peptides. Innovative methods of synthesis and characterization of membrane peptides are also presented. Part II, Application, presents a variety of specific applications for synthetic peptides. The collected protocols demonstrate the systematic manner in which peptides can be constructed, analyzed, and applied to attack contemporary problems in biochemistry, and this book will be an indispensable aid in the pursuit of new directions in peptide research.
700	1		_aFields, Gregg B _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617376375
776	0	8	_iPrinted edition: _z9781588295507
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-430-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2007
999			_c234519 _d234519