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003			ES-VaUE
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008			161103s2017 xxu| s |||| 0|eng d
020			_a9781493966707
024	7		_a10.1007/978-1-4939-6670-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aOncogene-Induced Senescence _bMethods and Protocols _cedited by Mikhail A. Nikiforov.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 220 páginas) _b32 ilustraciones, 23 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1534
505	0		_aThe Immortal Senescence -- Senescence Phenotypes Induced by Ras in Primary Cells -- Cellular Model of p21-Induced Senescence -- Detecting Markers of Therapy-Induced Senescence in Cancer Cells -- Genome-Wide miRNA Screening for Genes Bypassing Oncogene-Induced Senescence -- Detection of Dysfunctional Telomeres in Oncogene-Induced Senescence -- RT-qPCR Detection of Senescence-Associated Circular RNAs -- Autophagy Detection During Oncogene-Induced Senescence Using Fluorescence Microscopy -- Detecting the Senescence-Associated Secretory Phenotype (SASP) by High Content Microscopy Analysis -- Sudan Black B, the Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells -- Using [U-13C6]-Glucose Tracer to Study Metabolic Changes in Oncogene-Induced Senescence Fibroblasts -- Detection of the Ubiquitinome in Cells Undergoing Oncogene-Induced Senescence -- Detection of Reactive Oxygen Species in Cells Undergoing Oncogene-Induced Senescence -- Detection of Senescent Cells by Extracellular Markers Using a Flow Cytometry-Based Approach -- Metabolic Changes Investigated by Proton NMR Spectroscopy in Cells Undergoing Oncogene-Induced Senescence -- Detection of Nucleotide Disbalance in Cells Undergoing Oncogene-Induced Senescence -- Senescence-Like Phenotypes in Human Nevi -- Detection of Oncogene-Induced Senescence In Vivo -- Detection of Senescence Markers During Mammalian Embryonic Development -- Induction and Detection of Oncogene-Induced Cellular Senescence in Drosophila.
520			_aThis book offers in a single volume a unique collection of the state-of-the-art experimental procedures utilized for the induction, detection, and modeling of this complex cellular program of oncogene-induced senescence. The book encompasses protocols for studying this multi-step program in human specimens and a variety of experimental models including cultured mammalian cells, laboratory mice, and Drosophila melanogaster, as well as offering a description of high throughput approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Oncogene-Induced Senescence: Methods and Protocols represents a valuable asset for a wide audience of medical oncologists and researchers in the fields of oncology, molecular and cellular biology, biochemistry, and animal development.
700	1		_aNikiforov, Mikhail A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493966684
776	0	8	_iPrinted edition: _z9781493966691
776	0	8	_iPrinted edition: _z9781493982677
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6670-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234523 _d234523