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008			100301s2005 xxu| s |||| 0|eng d
020			_a9781592598779
024	7		_a10.1385/1592598773 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPeptide Synthesis and Applications _cedited by John Howl.
250			_a1st edition 2005
264		1	_aTotowa, NJ _bHumana Press _c2005
300			_a1 recurso en línea (X, 262 páginas) _b69 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v298
505	0		_aCommon Strategies -- Fundamentals of Modern Peptide Synthesis -- Chimerism -- Synthetic Methodologies and Applications -- Modification of Peptides and Other Drugs Using Lipoamino Acids and Sugars -- Synthesis of Linear, Branched, and Cyclic Peptide Chimera -- Synthesis of Cell-Penetrating Peptides for Cargo Delivery -- Incorporation of Phosphotyrosyl Mimetic 4- (Phosphonodifluoromethyl)phenylalanine (F2Pmp) Into Signal Transduction-Directed Peptides -- Expressed Protein Ligation for Protein Semisynthesis and Engineering -- Cellular Delivery of Peptide Nucleic Acid by Cell-Penetrating Peptides -- Quenched Fluorescent Substrate- Based Peptidase Assays -- A Convenient Method for Synthesis of Cyclic Peptide Libraries -- High-Throughput Peptide Synthesis -- Backbone Amide Linker Strategies for the Solid-Phase Synthesis of C - Terminal Modified Peptides -- 13 Synthesis of Peptide Bioconjugates -- Practical Guides -- Protein Identification by Mass Spectrometric Analyses of Peptides -- Manual Solid Phase Synthesis of Glutathione Analogs.
520			_aPeptide synthesis provides novel materials that can serve as both valuable research tools and candidate therapeutics. In Peptide Synthesis and Applications, a panel of expert researchers and established group leaders describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate numerous applications that employ peptides as unique and essential materials. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Peptide Synthesis and Applications offers novice and experienced investigators alike powerful techniques for the design and manufacture of their own peptide-based materials that can be used as productive research tools or developed as promising new therapeutics.
700	1		_aHowl, John _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617374906
776	0	8	_iPrinted edition: _z9781588293176
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592598773 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2005
999			_c234524 _d234524