000			04080nam a22003615i 4500
001			234526
003			ES-VaUE
005			20221220020608.0
007			cr nn 008mamaa
008			150709s2015 xxu| s |||| 0|eng d
020			_a9781493927425
024	7		_a10.1007/978-1-4939-2742-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aELISA _bMethods and Protocols _cedited by Robert Hnasko.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (X, 216 páginas) _b55 ilustraciones, 46 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1318
505	0		_aThe Biochemical Properties of Antibodies and their Fragments -- Hybridoma Technology -- Affinity Purification of Antibodies -- Bio conjugation of Antibodies to Horseradish Peroxidase (HRP). - Indirect ELISA -- Direct ELISA -- A Double-Sandwich ELISA for Identification of Monoclonal Antibodies Suitable for Sandwich Immunoassays -- ELISpot and DC-ELISspot Assay to Measure Frequency of Antigen-Specific IFNg Secreting Cells -- The Western Blot -- Development of Flow Through Assay for Detection of Antibodies to Taenia solium Using Protein-A Colloidal Gold Conjugate as a Probe.-Multiplexed Microsphere Suspension Array-Based Immunoassays -- Multiplex Immunoassay: A Planar Array on a Chip using the MagArray™ Technology -- Lateral Flow Immunoassay -- Immuno-PCR Assay for Sensitive Detection of Proteins in Real Time -- In-situ Proximity Ligation Assay (PLA) -- Tyramide Signal Amplification for Immunofluorescent Enhancement -- Correlative microscopy for Localization of Proteins In Situ: Pre-Embedding Immuno-Electron Microscopy using FluoroNanogold, Gold Enhancement and Low-Temperature Resin -- tiplex ELISA using Oligonucleotide Tethered Antibodies -- Gas Plasma Surface Chemistry for Biological Assays.
520			_aThis volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample.  The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production.  An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies.  Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems.  The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions.  Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.
700	1		_aHnasko, Robert _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493927418
776	0	8	_iPrinted edition: _z9781493927432
776	0	8	_iPrinted edition: _z9781493953851
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2742-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c234526 _d234526