000			04199nam a22003615i 4500
001			234531
003			ES-VaUE
005			20221220020608.0
007			cr nn 008mamaa
008			100715s2009 xxu| s |||| 0|eng d
020			_a9781597452076
024	7		_a10.1007/978-1-59745-207-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMycobacteria Protocols _cedited by Tanya Parish, Amanda Claire Brown.
250			_a2nd edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XIX, 456 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v465
505	0		_aIsolation of Mycobacterium Species Genomic DNA -- Isolation of Mycobacterial RNA -- Lipoglycans of Mycobacterium tuberculosis: Isolation, Purification, and Characterization -- Analyzing Lipid Metabolism: Activation and ?-Oxidation of Fatty Acids -- Analysis of Lipid Biosynthesis and Location -- Whole Genome Analysis Using Microarrays -- Use of DNA Microarrays to Study Global Patterns of Gene Expression -- Two-Dimensional Gel Electrophoresis-Based Proteomics of Mycobacteria -- Transport Assays and Permeability in Pathogenic Mycobacteria -- Continuous Culture of Mycobacteria -- Measuring Minimum Inhibitory Concentrations in Mycobacteria -- Rapid Screening of Inhibitors of Mycobacterium tuberculosis Growth Using Tetrazolium Salts -- Electroporation of Mycobacteria -- Ins and Outs of Mycobacterial Plasmids -- The Use of Temperature-Sensitive Plasmids in Mycobacteria -- Heterologous Expression of Genes in Mycobacteria -- Inducible Expression Systems for Mycobacteria -- Assaying Promoter Activity Using LacZ and GFP as Reporters -- Construction of Unmarked Deletion Mutants in Mycobacteria -- Construction of Targeted Mycobacterial Mutants by Homologous Recombination -- Phage Transposon Mutagenesis -- Gene Essentiality Testing in Mycobacterium smegmatis Using Specialized Transduction -- Gene Switching and Essentiality Testing -- Insertion Element IS6110-Based Restriction Fragment Length Polymorphism Genotyping of Mycobacterium tuberculosis -- Typing Mycobacterium tuberculosis Using Variable Number Tandem Repeat Analysis -- Molecular Detection of Drug-Resistant Mycobacterium tuberculosis with a Scanning-Frame Oligonucleotide Microarray -- MycoDB: An Online Database for Comparative Genomics of the Mycobacteria and Related Organisms -- Environmental Amoebae and Mycobacterial Pathogenesis.
520			_aDue to the rising threat of tuberculosis and other mycobacterial infections, methods to study the biology of the mycobacteria and to improve diagnostic, therapeutic and preventative reagents are still very much in need. Mycobacteria Protocols, Second Edition updates and refines the methods of the well-received first edition while adding newly developed methods culled from the most cutting-edge research in the field. Ranging from the basics of sub-cellular fractionation to advanced methods using specialized growth conditions and whole genome, transcriptome and proteome analysis, the chapters conform to the Methods in Molecular Biology™ series format, providing step-by-step laboratory protocols, lists of necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Mycobacteria Protocols, Second Edition is the perfect resource to promote and stimulate further research into these intriguing, important and most fractious of bacteria.
700	1		_aParish, Tanya _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBrown, Amanda Claire _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617378263
776	0	8	_iPrinted edition: _z9781588298898
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-207-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2009
999			_c234531 _d234531