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020 _a9781592590537
024 7 _a10.1385/1592590535
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aTransforming Growth Factor-Beta Protocols
_cedited by Philip H. Howe.
250 _a1st edition 2000
264 1 _aTotowa, NJ
_bHumana Press
_c2000
300 _a1 recurso en línea (X, 165 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v142
505 0 _aIn Vitro Assays for Measuring TGF? Growth Stimulation and Inhibition -- Measurements of Active TGF? Generated by Culture Cells -- Methods for Iodination of Active TGF?, TGF? Receptor Crosslinking and Immunoprecipitation of TGF?-Receptor Complexes -- Detection of TGF? in Body Fluids and Tissues -- Analysis of TGF?-Mediated Synthesis of Extracellular Matrix Components -- Characterization of SMAD Phosphorylation and SMAD/Receptor Interaction -- Promoter Analysis of TGF?-Responsive Genes by Transient Transfection and Deletional/Mutational Analysis -- Regulation of AP-1 Activity by TGF? -- CDK Pathway: Cyclin-Dependent Kinases (cdks) and Cyclin-Dependent Kinase Inhibitors -- Activation of the Mitogen-Activated Protein Kinase Pathway by TGF? -- 11 Detection of TGF? Type II Receptor Hotspot Mutations: The BAT-RII Assay -- Mutation Detection in the TGF? Receptors and SMAD Genes: RT-PCR and Sequencing -- Analysis of TGF?-Inducible Apoptosis.
520 _aThe recent isolation and identification of the TGFß receptors, as well as their intracellular mediating proteins, has driven the development of many new methodologies and techniques for the study of TGFß. In Transforming Growth Factor-Beta Protocols, Philip H. Howe and a group of well-versed experimentalists present the first major collection of indispensable classic and cutting-edge TGFß assays. Described in great detail to ensure robust and successful results, these readily reproducible techniques range from the growth inhibition assay for TGFß to methodologies for monitoring its interactions with the mediating proteins. Extensive notes discuss potential pitfalls and provide tips on how to avoid failures, and throughout, emphasis is given to detailing those technical steps critical for experimental success that are often omitted in the primary literature. Concise and highly practical, Transforming Growth Factor-Beta Protocols provides today's molecular and cell biologists-both expert and novice-with time-tested methods for the identification and analysis of the signal transduction pathways by which TGFß induces and modulates physiological behavior.
700 1 _aHowe, Philip H
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781489943989
776 0 8 _iPrinted edition:
_z9780896036468
776 0 8 _iPrinted edition:
_z9781489943972
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590535
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2000
999 _c234536
_d234536