000			04370nam a22003495i 4500
001			234541
003			ES-VaUE
005			20221220020609.0
007			cr nn 008mamaa
008			100301s2007 xxu| s |||| 0|eng d
020			_a9781597452670
024	7		_a10.1385/159745267X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aProtein Phosphatase Protocols _cedited by Greg Moorhead.
250			_a1st edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XV, 387 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v365
505	0		_aAnalysis of Protein Phosphatases -- A Brief Introduction to the Protein Phosphatase Families -- Small-Molecule Inhibitors of Ser/Thr Protein Phosphatases -- Synthesis and Use of the Protein Phosphatase Affinity Matrices Microcystin-Sepharose and Microcystin-Biotin-Sepharose -- Utilizing Protein Phosphatase Inhibitors to Define PP2A as a Regulator of Ataxia-Telangiectasia Mutated -- An Automated Fluorescence-Based Method for Continuous Assay of PP2A Activity -- An In Vivo Assay to Quantify Stable Protein Phosphatase 2A (PP2A) Heterotrimeric Species -- Mutagenesis and Expression of the Scaffolding A? and A? Subunits of PP2A -- Isolation and Characterization of PP2A Holoenzymes Containing FLAG-Tagged B Subunits -- Purification of PP2A Holoenzymes by Sequential Immunoprecipitation with Anti-Peptide Antibodies -- Purification of PP2Ac from Bovine Heart -- Visualization of Intracellular PP1 Targeting Through Transiently and Stably Expressed Fluorescent Protein Fusions -- Yeast Two-Hybrid Screens to Identify Drosophila PP1-Binding Proteins -- Identification of Cellular Protein Phosphatase-1 Regulators -- Assay for Three-Way Interaction of Protein Phosphatase-1 (Glc7) With Regulatory Subunits Plus Phosphatase Inhibitor-2 -- Phosphorylation of the Protein Phosphatase Type 1 Inhibitor Protein CPI-17 by Protein Kinase C -- Purification of Smooth Muscle Myosin Phosphatase Using a Thiophosphorylated Myosin Light-Chain-Affinity Resin -- Proteins Interacting With Saccharomyces cerevisiae Type 1 Protein Phosphatase Catalytic Subunit Identified by Single-Step Affinity Purification and Mass Spectrometry -- Expression of Protein Histidine Phosphatase in Escherichia coli, Purification, and Determination of Enzyme Activity -- The Use of RNA Interference to Analyze Protein Phosphatase Function in Mammalian Cells -- Recognition of a PP2C Interaction Motif in Several Plant Protein Kinases -- Use of Yeast Genetic Tools to Define Biological Roles of Novel Protein Phosphatases -- Targeting of PP2C in Budding Yeast -- Phosphatase Targets in TOR Signaling -- Functional Characterization of Small CTD Phosphatases -- Genome-Scale Discovery and Characterization of Class-Specific Protein Sequences -- Yeast Substrate-Trapping System for Isolating Substrates of Protein Tyrosine Phosphatases.
520			_aProtein Phosphatase Protocols presents a broad range of protocols for the study of protein phosphatases, all written by experts and innovators from phosphatase laboratories around the world. This volume is a compendium of resources for the study of protein phosphatases and their potential as drug targets. Experimental methodologies are taken from proteomics, bioinformatics, genomics, biochemistry, RNAi, and genetics. Included protocols utilize several model organisms, including yeast, Drosophila, and various plant and human cells. Protein Phosphatase Protocols provides a concise introduction to protein phosphatase research, with particular attention to phosphatase families. Assays for identification, quantification, purification, and functional characterization are presented throughout in specific detail.
700	1		_aMoorhead, Greg _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617377334
776	0	8	_iPrinted edition: _z9781588297112
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159745267X _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2007
999			_c234541 _d234541