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020 _a9781592592340
024 7 _a10.1385/1592592341
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aDNA Arrays
_bMethods and Protocols
_cedited by Jang B. Rampal.
250 _a1st edition 2001
264 1 _aTotowa, NJ
_bHumana Press
_c2001
300 _a1 recurso en línea (XI, 264 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v170
505 0 _aDNA Microarrays -- Gel-Immobilized Microarrays of Nucleic Acids and Proteins -- Sequencing by Hybridization Arrays -- Ethical Ramifications of Genetic Analysis Using DNA Arrays -- Photolithographic Synthesis of High-Density Oligonucleotide Arrays -- Automated Genotyping Using the DNA MassArray™ Technology -- Ink-Jet-Deposited Microspot Arrays of DNA and Other Bioactive Molecules -- Printing DNA Microarrays Using the Biomek® 2000 Laboratory Automation Workstation -- Hybridization Analysis of Labeled RNA by Oligonucleotide Arrays -- Analysis of Nucleic Acids by Tandem Hybridization on Oligonucleotide Microarrays -- DNA Sequencing by Hybridization with Arrays of Samples or Probes -- Using Oligonucleotide Scanning Arrays to Find Effective Antisense Reagents -- Low-Resolution Typing of HLA-DQA1 Using DNA Microarray -- Gene Expression Analysis on Medium-Density Oligonucleotide Arrays -- Use of Bioinformatics in Arrays -- Confocal Scanning of Genetic Microarrays -- Business Aspects of Biochip Technologies.
520 _aMicroarray technology, which permits the rapid, simultaneous, and highly sensitive analysis of large numbers of biological samples, is now coming into widespread use for advanced research on gene expression, mutation analysis, proteomics, and gene sequencing. In DNA Arrays: Methods and Protocols, Jang Rampal and a authoritative panel of researchers, engineers, and technologists explain in detail how to design and construct these DNA arrays, as well as how to hybridize them with biological samples for analysis. In step-by-step instructions these experts detail not only how to attach or print arrays on various matrices, but also biological sample preparation (DNA and RNA), hybridization conditions, signal detection, probe optimization, different printing technologies, and data collection and analysis (bioinformatics). Additional topics covered include genotyping, sequencing by hybridization, antisense reagents, HLA-DQA typing techniques, and gene expression analysis. Rounding out the technical presentation are three chapters that review the history of microarrays, the ethical ramifications of genetic analysis using DNA arrays, and the business aspects of biochip technologies. Forward-looking and state-of-the-art, DNA Arrays: Methods and Protocols provides all investigators engaged in biological and biomedical research the full range of effective, readily reproducible microarray techniques needed today to analyze on a large scale the many different genes and gene sequences now available from the Human Genome Project.
700 1 _aRampal, Jang B
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617372049
776 0 8 _iPrinted edition:
_z9780896038226
776 0 8 _iPrinted edition:
_z9781489944009
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592341
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2001
999 _c234542
_d234542