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008			130109s2013 xxu| s |||| 0|eng d
020			_a9781627032452
024	7		_a10.1007/978-1-62703-245-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aBacterial Cell Surfaces _bMethods and Protocols _cedited by Anne H. Delcour.
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XII, 399 páginas) _b66 ilustraciones, 16 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v966
505	0		_aA Bird's Eye View of the Bacterial Landscape -- Visualizing the Bacterial Cell Surface: An Overview -- Purification of the Outer Membrane Usher Protein and Periplasmic Chaperone-Subunit Complexes from the P and Type 1 Pilus Systems -- Experimental Manipulation of the Microbial Functional Amyloid Called Curli -- Visualization of Gram-Positive Bacterial Pili -- Single Cell Microfluidic Studies of Bacterial Motility -- Chromatographic Analysis of the Escherichia coli Polysialic Acid Capsule -- Analysis of Exopolysaccharides in Myxococcus xanthus Using Confocal Laser Scanning Microscopy -- Assessment of Multidrug Efflux Assemblies by Surface Plasmon Resonance -- Fluorescence Microscopy and Proteomics to Investigate Subcellular Localization, Assembly, and Function of the Type II Secretion System -- Pore Formation by T3SS Translocators: Liposome Leakage Assay -- Isolation of Bacterial Type IV Machine Subassemblies -- Production and Crystallization of Bacterial Type V Secretion Proteins -- Assembly of Bacterial Outer Membrane Proteins -- The Outer Membrane of Gram-Negative Bacteria: Lipid A Isolation and Characterization -- Quantitative and Qualitative Preparations of Bacterial Outer Membrane Vesicles -- In Vitro Peptidoglycan Synthesis Assay with Lipid II Substrate -- Extraction of Cell Wall-Bound Teichoic Acids and Surface Proteins from Listeria monocytogenes -- Subfractionation and Analysis of the Cell Envelope (Lipo)polysaccharides of Mycobacterium tuberculosis -- Protein Disulfide Bond Formation in the Periplasm: Determination of the In Vivo Redox State of Cysteine Residues -- Using Reporter Genes and the Escherichia coli ASKA Over-Expression Library in Screens for Regulators of the Gram Negative Envelope Stress Response -- Isolation of Bacteria Envelope Proteins -- Patch Clamp Electrophysiology for the Study of Bacterial Ion Channels in Giant Spheroplasts of E. coli -- Electrophysiological Characterization of Bacterial Pore-Forming Proteins in Planar Lipid Bilayers.
520			_aIn recent years, molecular microbiology has emerged as a top, cutting-edge biological discipline, thanks to the multi-disciplinary and integrative approaches taken by investigators seeking to understand the intricacies of the microbial world and how it affects human health and the biosphere. In Bacterial Cell Surfaces: Methods and Protocols, recent advances in structural biology, proteomics, and imaging techniques, together with the traditional biochemical and genetic approaches, are provided in order to present an exciting look into the structure, function, and regulation of the bacterial cell envelope. The detailed volume contains examples of traditional and innovative tools for the study of protein structure and function and enzymatic activities, the purification and analysis of macromolecules and their complexes, and the investigation of regulatory mechanisms and cell biological processes.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Bacterial Cell Surfaces: Methods and Protocols is aimed at the microbiologist, biochemist, or cell biologist, whether a beginning graduate student or a veteran experimentalist, who wishes to learn new methodologies and take advantage of the years of research and protocol optimization from the best laboratories around the world.
700	1		_aDelcour, Anne H _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627032445
776	0	8	_iPrinted edition: _z9781627032469
776	0	8	_iPrinted edition: _z9781493959938
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-245-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c234543 _d234543