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020 _a9781592592159
024 7 _a10.1385/1592592155
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aGene Targeting Protocols
_cedited by Eric B. Kmiec.
250 _a1st edition 2000
264 1 _aTotowa, NJ
_bHumana Press
_c2000
300 _a1 recurso en línea (XV, 233 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v133
505 0 _aNucleic Acid Transfer Using Cationic Lipids -- Optimizing Polyethylenimine-Based Gene Transfer into Mammalian Brain for Analysis of Promoter Regulation and Protein Function -- Gene Transfer and Drug Delivery by Electronic Pulse Delivery -- Strategies for Improving the Frequency and Assessment of Homologous Recombination -- Effective Gene Transfer Using Viral Vectors Based on SV40 -- Rapid Generation of Isogenic Mammalian Cell Lines Expressing Recombinant Transgenes by Use of Cre Recombinase -- Site-Directed Alteration of Genomic DNA by Small-Fragment Homologous Replacement -- Mutation Correction by Homologous Recombination with an Adenovirus Vector -- Site-Specific Targeting of DNA Plasmids to Chromosome 19 Using AAV Cis and Trans Sequences -- Adeno-Associated Virus Based Gene Therapy in Skeletal Muscle -- Rapid Establishment of Myeloma Cell Lines Expressing Fab(Tac)-Protamine, a Targetable Protein Vector, Directed Against High-Affinity ?-Chain of Human Interleukin-2 Receptor -- EBV-Derived Episomes to Probe Chromatin Structure and Gene Expression in Human Cells -- Triplex-Directed Site-Specific Genome Modification -- Use of Quantitative Ligation-Mediated Polymerase Chain Reaction to Detect Gene Targeting by Alkylating Oligodeoxynucleotides -- Gene Targeting in Plants via Site-Directed Mutagenesis -- Antisense Oligonucleotides as Modulators of Pre-mRNA Splicing.
520 _aIn Gene Targeting Protocols, a panel of innovative investigators present in readily reproducible detail the latest techniques for gene replacement, gene knockout, and gene repair in both animals and plants. These expert researchers review the best gene transfer vehicles (liposomes, PEI, and EPD), discuss the parameters that affect vector uptake, and detail successful methods for gene targeting (Cre-ox, and adeno and adeno-associated viruses). The methods include two fundamentally different approaches to gene targeting- one using a fusion protein to deliver a vector to a virus for possible therapeutic effect, and one entailing an episomal-based Epstein-Barr vector that can modulate the chromatin assembly process, and thus overcome a serious barrier to therapeutic gene targeting. The book also has several techniques for using oligonucleotides in gene targeting, a methodology that may revolutionize the entire field. Innovative and wide ranging, Gene Targeting Protocols offers today's researchers robust and proven gene-targeting techniques that are essential to understanding and regulating biological processes at the genetic level.
700 1 _aKmiec, Eric B
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781489941589
776 0 8 _iPrinted edition:
_z9780896033603
776 0 8 _iPrinted edition:
_z9781489941572
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592155
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2000
999 _c234549
_d234549