000			04127nam a22003735i 4500
001			234551
003			ES-VaUE
005			20221220020609.0
007			cr nn 008mamaa
008			111214s2012 xxu| s |||| 0|eng d
020			_a9781617794803
024	7		_a10.1007/978-1-61779-480-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aProtein NMR Techniques _cedited by Alexander Shekhtman, David S. Burz.
250			_a3rd edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XIV, 518 páginas) _b107 ilustraciones, 23 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v831
505	0		_aA Novel Bacterial Expression Method with Optimized Parameters for Very High Yield Production of Triple-labeled Proteins -- Isotopic Labeling of Heterologous Proteins in the Yeast Pichia pastoris and Kluyveromyces lactis -- Isotope Labeling in Insect Cells -- Isotope Labeling in Mammalian Cells -- Cell-free Protein Production for NMR Studies -- Cell-free Membrane Protein Expression for Solid-state NMR -- Expression and Purification of Src-family Kinases for Solution NMR Studies -- NMR Studies of Large Protein Systems -- Protein Dynamics by 15N Nuclear Magnetic Relaxation -- Bacterial Production and Solution NMR Studies of a Viral Membrane Ion Channel -- Preparation of the Modular Multi-Domain Protein RPA for Study by NMR Spectroscopy -- NMR Studies of Protein-RNA Interactions -- Preparation and Optimization of Protein-DNA Complexes Suitable for Detailed NMR Studies -- NMR Studies of Protein-ligand Interactions -- In-cell NMR Spectroscopy in Escherichia coli -- Deuterated Peptides and Proteins: Structure and Dynamics Studies by MAS solid-state NMR -- Solid-State NMR Spectroscopy of Protein Complexes -- Synthesis, Purification and Characterization of Single Helix Membrane Peptides and Proteins for NMR Spectroscopy -- Assignment of Backbone Resonances in a Eukaryotic Protein Kinase - ERK2 -- Electrostatics of Hydrogen Exchange for Analyzing Protein Flexibility -- Fast Protein Backbone NMR Resonance Assignment using the BATCH Strategy -- Comprehensive Automation for NMR Structure Determination of Proteins -- ARIA for Solution and Solid-state NMR Protein NMR Techniques -- Determining Protein Dynamics from 15N Relaxation Data by using DYNAMICS.                   .
520			_aThe field of protein NMR spectroscopy has rapidly expanded into new areas of biochemistry, molecular biology and cell biology research that were impossible to study as recently as ten years ago. This third edition of Protein NMR Techniques, expands upon the previous editions with current, detailed authoritative but down-to-earth descriptions of new methodologies. These include techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Protein NMR Techniques, Third Edition, seeks to aid scientists in understanding the latest innovations in the field of protein NMR.
700	1		_aShekhtman, Alexander _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBurz, David S _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617794797
776	0	8	_iPrinted edition: _z9781617794810
776	0	8	_iPrinted edition: _z9781493961955
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-480-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2012
999			_c234551 _d234551