000			03512nam a22003735i 4500
001			234563
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007			cr nn 008mamaa
008			100301s2001 xxu| s |||| 0|eng d
020			_a9781592591138
024	7		_a10.1385/1592591132 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDNA Sequencing Protocols _cedited by Colin A Graham, Alison J.M. Hill.
250			_a2nd edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (X, 244 páginas) _b33 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v167
505	0		_ato DNA Sequencing -- The Universal Primers and the Shotgun DNA Sequencing Method -- M13 Sequencing -- Primer Design and Primer-Directed Sequencing -- Direct Sequencing of DNA Produced in a Polymerase Chain Reaction -- Solid Phase Fluorescent Sequencing of the CFTR Gene -- Shotgun DNA Sequencing -- Cycle Sequencing -- Cycle Sequencing of Polymerase Chain Reaction-Amplified Genomic DNA with Dye-Labeled Universal Primers -- Automated Fluorescent DNA Sequencing on the ABI PRISM 377 -- Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer -- Fluorescent Sequencing Protocols for the ALF -- Fluorescent Sequencing for Heterozygote Mutation Detection -- Sequence Databases and the Internet -- DNA Sequencing by Capillary Array Electrophoresis.
520			_aSince the publication of the first edition of DNA Sequencing Protocols in 1993, major advances in its technology have made PCR-based semiautomated fluorescent sequencing the norm and enabled the complete sequencing of complex genomes. In DNA Sequencing Protocols, 2nd Edn., Colin Graham and a team of leading investigators and expert clinical scientists update the first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. Each method provides detailed, step-by-step instructions to ensure successful results, and includes helpful notes identifying and addressing potential problems. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will impact today's genome sequencing projects, improve the accuracy and quality of DNA sequences obtained by smaller laboratories, and help lay the foundation for molecular diagnostics.
700	1		_aGraham, Colin A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aHill, Alison J.M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489942128
776	0	8	_iPrinted edition: _z9781489942111
776	0	8	_iPrinted edition: _z9780896037168
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591132 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2001
999			_c234563 _d234563