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020 _a9781607616467
024 7 _a10.1007/978-1-60761-646-7
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPlant Epigenetics
_bMethods and Protocols
_cedited by Igor Kovalchuk, Franz J. Zemp.
250 _a1st edition 2010
264 1 _aTotowa, NJ
_bHumana Press
_c2010
300 _a1 recurso en línea (X, 273 páginas)
_b51 ilustraciones, 12 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v631
505 0 _aAnalysis of DNA Methylation in Plants by Bisulfite Sequencing -- Analysis of Bisulfite Sequencing Data from Plant DNA Using CyMATE -- Analysis of Locus-Specific Changes in Methylation Patterns Using a COBRA (Combined Bisulfite Restriction Analysis) Assay -- Detection of Changes in Global Genome Methylation Using the Cytosine-Extension Assay -- In Situ Analysis of DNA Methylation in Plants -- Analysis of Mutation/Rearrangement Frequencies and Methylation Patterns at a Given DNA Locus Using Restriction Fragment Length Polymorphism -- Isoschizomers and Amplified Fragment Length Polymorphism for the Detection of Specific Cytosine Methylation Changes -- Analysis of Small RNA Populations Using Hybridization to DNA Tiling Arrays -- Northern Blotting Techniques for Small RNAs -- qRT-PCR of Small RNAs -- Cloning New Small RNA Sequences -- Genome-Wide Mapping of Protein-DNA Interaction by Chromatin Immunoprecipitation and DNA Microarray Hybridization (ChIP-chip). Part A: ChIP-chip Molecular Methods -- Genome-Wide Mapping of Protein-DNA Interaction by Chromatin Immunoprecipitation and DNA Microarray Hybridization (ChIP-chip). Part B: ChIP-chip Data Analysis -- Metaanalysis of ChIP-chip Data -- Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis -- cDNA Libraries for Virus-Induced Gene Silencing -- Detection and Quantification of DNA Strand Breaks Using the ROPS (Random Oligonucleotide Primed Synthesis) Assay -- Reporter Gene-Based Recombination Lines for Studies of Genome Stability -- Plant Transgenesis.
520 _aThe past fifteen years have witnessed major advances in epigenetics, one of the most popular and quickly evolving fields of modern science. In Plant Epigenetics: Methods and Protocols, expert researchers explore the most recent developments, examining in great detail the contribution of epigenetic regulation to cell function in plants. Chapters include a variety of protocols for studying the function of small non-coding RNAs, DNA methylation, and histone modifications in plants, often in different degrees of complexity. This volume describes bioinformatic approaches to the analysis of high-throughput data, such as bisulfite sequencing and Chip-on-chip assays. It features much-desired protocols for plant transgenesis and the analysis of genome stability, with a detailed discussion of their applications to epigenetic studies. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Wide-ranging and innovative, Plant Epigenetics: Methods and Protocols is an invaluable manual designed to help researchers uncover the undiscovered and unexplained phenomena in plant biology.
700 1 _aKovalchuk, Igor
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aZemp, Franz J
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781607616504
776 0 8 _iPrinted edition:
_z9781607616450
776 0 8 _iPrinted edition:
_z9781493957569
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-646-7
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2010
999 _c234569
_d234569