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008			161118s2017 xxu| s |||| 0|eng d
020			_a9781493964697
024	7		_a10.1007/978-1-4939-6469-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Hormones _bMethods and Protocols _cedited by Jürgen Kleine-Vehn, Michael Sauer.
250			_a3rd edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 288 páginas) _b117 ilustraciones, 106 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1497
505	0		_aReal Time Analysis of the Apical Hook Development -- Grafting with Arabidopsis thaliana -- Tips and Tricks for Exogenous Application of Synthetic Post-Translationally Modified Peptides to Plants -- Assaying Germination and Seedling Responses of Arabidopsis to Karrikins -- Low-cost Microprocessor-Controlled Rotating Stage for Medium-Throughput Time-Lapse Plant Phenotyping -- Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research -- High throughput scoring of Seed Germination -- Histochemical Staining of β-Glucuronidase and its Spatial Quantification -- Imaging TCSn::GFP, A Synthetic Cytokinin Reporter, in Arabidopsis thaliana -- Highlighting Gibberellins Accumulation Sites in Arabidopsis Thaliana Root Using Fluorescently Labeled Gibberellins -- In-silico Methods for Cell Annotation, Quantification of Gene Expression and Cell Geometry at Single Cell Resolution using 3DCellAtlas -- Analysing Cell Wall Elasticity after Hormone Treatment: An example using Tobacco BY-2 Cells and Auzin -- FRET-FLIM for Visualizing and Quantifying Protein Interactions in Live Plant Cells -- In vivo Identification of Plant Protein Complexes using IP-MS/MS -- Assaying auxin Receptor Activity using SPR assays with F-Box Proteins and Aux-IAA degrons -- Studying Transcription Factor Binding To Specific Genomic Loci by Chromatin Immunoprecipitation (ChIP) -- Hormone Receptor Glycosylation -- Highly Sensitive Salicylic Acid Quantification in Milligram Amounts of Plant Tissue -- High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana -- Hormone Profiling in Plant Tissues -- Use of Xenopus laevis Oocytes to Study Auxin Transport -- Characterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometry. .
520			_aThis volume aims to present a representative cross-section of modern experimental approaches relevant to Plant Hormone Biology, ranging from relatively simple physiological to highly sophisticated methods. Chapters describe physiological, developmental, microscopy-based techniques, measure hormone contents, and heterologous systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Hormones:Methods and Protocols, Third Edition aims to provide researchers with useful methods to advance their research.
700	1		_aKleine-Vehn, Jürgen _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSauer, Michael _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493964673
776	0	8	_iPrinted edition: _z9781493964680
776	0	8	_iPrinted edition: _z9781493982103
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6469-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234571 _d234571