000			03851nam a22003735i 4500
001			234574
003			ES-VaUE
005			20221220020611.0
007			cr nn 008mamaa
008			160924s2016 xxu| s |||| 0|eng d
020			_a9781493964338
024	7		_a10.1007/978-1-4939-6433-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRNA Structure Determination _bMethods and Protocols _cedited by Douglas H. Turner, David H. Mathews.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XV, 283 páginas) _b81 ilustraciones, 66 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1490
505	0		_aCrumple: An Efficient Tool to Explore thoroughly the RNA Folding Landscape -- Secondary Structure Prediction of Single Sequences using RNAstructure -- Prediction of Secondary Structures Conserved in Multiple RNA Sequences -- Predicting RNA-RNA Interactions Using RNAstructure -- A Method to Predict the Structure and Stability of RNA/RNA Complexes -- STarMir Tools for Prediction of microRNA Binding Sites -- Traditional Chemical Mapping of RNA Structure in vitro and in vivo -- High-throughput Nuclease Probing of RNA Structures using FragSeq -- Mapping RNA Structure in vitro with SHAPE Chemistry and Next Generation Sequencing (SHAPE-Seq) -- Experiment-assisted Secondary Structure Prediction with RNAstructure -- RNA Secondary Structure Determination by NMR -- Modeling Small Non-canonical RNA Motifs with the Rosetta FARFAR Server -- Automated RNA 3D Structure Prediction with RNAComposer -- RNA 3D Structure Modeling by Combination of Template-Based Method ModeRNA, Template-Free Folding with SimRNA, and Refinement with QRNAS -- Exploring Alternative RNA Structure Sets using MC-FLashfold and db2cm -- NMR Methods for Characterization of RNA Secondary Structure -- The Quick and the Dead: A Guide to Fast Phasing of Small Ribozyme and Riboswitch Crystal Structures. .
520			_aThis volume provides protocols and procedures for determining and modeling RNA structure. Chapters guide the reader through protocols for RNA secondary structure prediction, single sequence modeling, Crumple, RNAstructure to model conserved secondary structures with multiple homologs, the prediction of bimolecular secondary structures with RNAstructure, STarMir, protocols for structure mapping, mapping data to constrain or restrain RNA secondary structure prediction with RNAstructure, unassigned NMR resonances, modeling protocols for Rosetta FARFAR, RNAComposer , ModeRNA, and MC-Fold. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. < Authoritative and Practical, RNA Structure Determination: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aTurner, Douglas H _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMathews, David H _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493964314
776	0	8	_iPrinted edition: _z9781493964321
776	0	8	_iPrinted edition: _z9781493981984
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6433-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234574 _d234574