000			04038nam a22003615i 4500
001			234578
003			ES-VaUE
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007			cr nn 008mamaa
008			170915s2018 xxu| s |||| 0|eng d
020			_a9781493972586
024	7		_a10.1007/978-1-4939-7258-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSelenoproteins _bMethods and Protocols _cedited by Laurent Chavatte.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XV, 340 páginas) _b63 ilustraciones, 35 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1661
505	0		_aSECISearch3 and Seblastian: In Silico Tools to Predict SECIS Elements and Selenoproteins -- Selenoprofiles: A Computational Pipeline for Annotation of Selenoproteins -- SelGenAmic: An Algorithm for Selenoprotein Gene Assembly -- Selenocysteine tRNA[Ser]Sec, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification and Sequencing -- Identification and Characterization of Proteins that Bind to Selenoprotein 3' UTRs -- Specific Chemical Approaches for Studying Mammalian Ribosomes Complexed with Ligands Involved in Selenoprotein Synthesis -- Studying Selenoprotein mRNA Translation using RNA-Seq and Ribosome Profiling -- Modification of Selenoprotein mRNAs by Cap Tri-methylation -- Total Selenium Quantification in Biological Samples by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) -- Quantification of SeMet and SeCys in Biological Fluids and Tissues by Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry (HPLC - ICP MS) -- Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum -- Radioactive 75Se Labeling and Detection of Selenoproteins -- Non-radioactive Isotopic Labeling and Tracing of Selenoproteins in Cultured Cell Lines -- Detection of Selenoproteins by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP MS) in Immobilized pH Gradient (IPG) Strips -- Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues -- Overexpression of Recombinant Selenoproteins in E. coli -- Preparation of Selenocysteine-containing Forms of Human SELENOK and SELENOS -- Selenocysteine Mediated Expressed Protein Ligation of SELENOM -- Monitoring of Methionine Sulfoxide Content and Methionine Sulfoxide Reductase Activity -- Selective Evaluation of Thioredoxin Reductase Enzymatic Activities -- Association of Single Nucleotide Polymorphisms in Selenoprotein Genes with Cancer Risk -- Identification of Genetic Disorders Causing Disruption of Selenoprotein Biosynthesis. .
520			_aThis volume aims at providing an update on state of the art methodologies to study various aspects of selenoprotein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Selenoproteins: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aChavatte, Laurent _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493972579
776	0	8	_iPrinted edition: _z9781493972593
776	0	8	_iPrinted edition: _z9781493984336
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7258-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c234578 _d234578