000			03335nam a22003615i 4500
001			234583
003			ES-VaUE
005			20221220020611.0
007			cr nn 008mamaa
008			131028s2014 xxu| s |||| 0|eng d
020			_a9781627037037
024	7		_a10.1007/978-1-62703-703-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_amiRNA Maturation _bMethods and Protocols _cedited by Christoph Arenz.
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (X, 192 páginas) _b50 ilustraciones, 29 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1095
505	0		_aThe Pathway of miRNA Maturation -- Micro RNA Maturation and Human Disease -- Approaches to the Modulation of miRNA Maturation -- Expression Profiling of Components of the miRNA Maturation Machinery -- Primary microRNA Processing Assay Reconstituted using Recombinant Drosha and DGCR8 -- In-vivo processing Assay Based on a Dual Luciferase Reporter System to Evaluate DROSHA Enzymatic Activity -- Assaying Dicer-mediated miRNA Maturation by Means of Fluorescent Substrates -- A Fluorescence Correlation Spectroscopy-based Enzyme Assay for Human Dicer -- Detection of Micro RNA Maturation Using Unmodified pre-miRNA and Branched Rolling Circle Amplification -- Identification of Inhibitors of microRNA Function from Small Molecule Screens -- Quantitative RT-PCR Specific for Precursor and Mature miRNAs -- Cellular MicroRNA Sensors Based on Luciferase Reporters -- Inhibition of miRNA maturation by Peptide Nucleic Acids -- Molecular Methods for Validation of the Biological Activity of Peptide Nucleic Acids Targeting MicroRNAs -- Lentiviral Overexpression of miRNAs.
520			_aIn miRNA Maturation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study miRNA maturation. These included established methods such as fluorescent and non-fluorescent methods for homogenous assays of Dicer-mediated miRNA maturation or an in vivo assay for Drosha activity. Moreover, the volume also contains useful, but less-common methods that are hard to find elsewhere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, miRNA Maturation: Methods and Protocols seeks to widen the view on miRNA as biological mediator and potential drug target.
700	1		_aArenz, Christoph _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627037044
776	0	8	_iPrinted edition: _z9781627037020
776	0	8	_iPrinted edition: _z9781493960163
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-703-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c234583 _d234583