000			04911nam a22003615i 4500
001			234588
003			ES-VaUE
005			20221220020612.0
007			cr nn 008mamaa
008			110512s2011 xxu| s |||| 0|eng d
020			_a9781617791024
024	7		_a10.1007/978-1-61779-102-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMicrobial Toxins _bMethods and Protocols _cedited by Otto Holst.
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XI, 215 páginas) _b53 ilustraciones, 3 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v739
505	0		_aBacterial Protein Toxins -- Detection of Bacterial Protein Toxins by Solid Phase Magnetic Immunocapture and Mass Spectrometry. Sensitive and Rapid Detection of Cholera Toxin-Producing Vibrio cholerae using Loop-Mediated Isothermal Amplification. Ultra Sensitive Detection of Botulinum Neurotoxins and Anthrax Lethal Factor in Biological Samples by ALISSA. Examination of Bacillus anthracis Spores by Multiparameter Flow Cytometry. A Cell-Based Fluorescent Assay to Detect the Activity of Shiga Toxin and Other Toxins That Inhibit Protein Synthesis. Use of a Vero Cell-Based Fluorescent Assay to Assess Relative Toxicities of Shiga Toxin 2 Subtypes from Escherichia coli. Molecular Methods - Chip Assay and Quantitative Real-Time PCR - in Detecting Hepatotoxic Cyanobacteria. Endotoxins -- Capillary Electrophoresis Chips for Fingerprinting Endotoxin Chemotypes from Whole-Cell Lysates. Isolation of Smooth-Type Lipopolysaccharides to Electrophoretic Homogeneity. A Method for Unobtrusive Labeling of Lipopolysaccharides with Quantum Dots. Fluorescence-Based Methods to Assay Inhibitors of Lipopolysaccharide Synthesis. Micromethods for Lipid A Isolation and Structural Characterization. Two Efficient Methods for the Conjugation of Smooth-Form Lipopolysaccharides (LPS) with Probes Bearing Hydrazine or Amino Groups. I. LPS Activation with Cyanogen Bromide. Two Efficient Methods for the Conjugation of Smooth-Form Lipopolysaccharides (LPS) with Probes Bearing Hydrazine or Amino Groups. II. LPS Activation with a Cyanopyridinium Agent. Mold Fungus Toxins -- Extraction and Analysis of Fumonisins and Compounds Indicative of Fumonisin Exposure in Plant and Mammalian Tissues and Cultured Cells. Determination of Fumonisins B1 and B2 in Maize Food Products by a New Analytical Method Based on High-Performance Liquid Chromatography and Fluorometric Detection with Post-Column Derivatization. A Confirmatory Method for Aflatoxin M1 Determination in Milk Based on Immunoaffinity Clean-Up and High-Performance Liquid Chromatography with Fluorometric Detection. Simultanious Determination of Aflatoxins B1, B2, G1 and G2 in Foods and Feed Materials by High-Performance Liquid Chromatography with On-Line Photochemical Derivatization and Fluorescence Detection. Highly Sensitive PCR-Based Detection Specific to Aspergillus flavus.A Rapid Enzymatic Method for Aflatoxin B Detection.
520			_aInterest in the field of microbial toxins is ever growing and spreading across a broad spectrum of scientific disciplines. In an effort to supplement the available reference texts on toxins, Microbial Toxins: Methods and Protocols includes protocols on mold fungus toxins, with some focus on aflatoxins. Intended to support a wide variety of researchers, Microbial Toxins: Methods and Protocols presents the reader with biological, chemical, physical, and medical approaches, as well as state-of-the-art research techniques. Divided into three convenient sections, this detailed volume covers bacterial protein toxins, endotoxins, and mold fungus toxins. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Microbial Toxins: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential field.
700	1		_aHolst, Otto _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617791017
776	0	8	_iPrinted edition: _z9781617791031
776	0	8	_iPrinted edition: _z9781493961337
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-102-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2011
999			_c234588 _d234588