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001			234589
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007			cr nn 008mamaa
008			100715s2010 xxu| s |||| 0|eng d
020			_a9781603273459
024	7		_a10.1007/978-1-60327-345-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHistology Protocols _cedited by Tim D. Hewitson, Ian A. Darby.
250			_a1st edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (X, 230 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v611
505	0		_aTissue Processing -- Tissue Preparation for Histochemistry: Fixation, Embedding, and Antigen Retrieval for Light Microscopy -- An Optimized RNA Extraction Method from Archival Formalin-Fixed Paraffin-Embedded Tissue -- Laser-Capture Microdissection and Pressure Catapulting for the Analysis of Gene Expression in the Renal Glomerulus -- Staining Techniques -- Immunofluorescence Detection of the Cytoskeleton and Extracellular Matrix in Tissue and Cultured Cells -- Double Immunohistochemistry with Horseradish Peroxidase and Alkaline Phosphatase Detection Systems -- Retrogradely Transported Neuronal Tracers Combined with Immunohistochemistry Using Free-Floating Brain Sections -- High-Pressure Freezing, Chemical Fixation and Freeze-Substitution for Immuno-electron Microscopy -- Lectin Histochemistry for Light and Electron Microscopy -- Duplex In Situ Hybridization in the Study of Gene Co-regulation in the Vertebrate Brain -- Special Stains for Extracellular Matrix -- Active Staining of Mouse Embryos for Magnetic Resonance Microscopy -- Immunohistochemical Detection of Tumour Hypoxia -- In Situ Localization of Apoptosis Using TUNEL -- Imaging Techniques -- Use of Confocal Microscopy for Three-Dimensional Imaging of Neurons in the Spinal Cord -- High-Resolution Confocal Imaging in Tissue -- Software-Based Stacking Techniques to Enhance Depth of Field and Dynamic Range in Digital Photomicrography -- Image Analysis and Quantitative Morphology.
520			_aWith much of what we know about the pathogenesis of disease coming from the systematic and careful study of histological material, histology has proven to be an incredibly valuable area of study. In Histology Protocols, expert researchers in the field contribute detailed methods involving the preparation of tissue, providing an overview of fixation, embedding, and processing, along with the required techniques for the retrieval of RNA from histological sections, followed by sections on routine and specialist histological staining techniques, including immuno-, lectin, and hybridization histochemistry, histological staining, as well as specific methods for the in situ identification of hypoxia and apoptosis, and details on the advances in imaging and image analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Histology Protocols will provide molecular biologists with the basic histochemical techniques and histologists with the molecular techniques necessary to realize the potential of their resource.
700	1		_aHewitson, Tim D _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aDarby, Ian A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607614791
776	0	8	_iPrinted edition: _z9781603273442
776	0	8	_iPrinted edition: _z9781493956944
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-345-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2010
999			_c234589 _d234589