| 000 | 02560nam a22003495i 4500 | ||
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| 001 | 234595 | ||
| 003 | ES-VaUE | ||
| 005 | 20221220020612.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 120327s2012 xxu| s |||| 0|eng d | ||
| 020 | _a9781617797675 | ||
| 024 | 7 |
_a10.1007/978-1-61779-767-5 _2doi |
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| 040 |
_aES-MaUEC _bspa _cES-MaUEC |
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| 245 | 1 | 0 |
_aExon Skipping _bMethods and Protocols _cedited by Annemieke Aartsma-Rus. |
| 250 | _a1st edition 2012 | ||
| 264 | 1 |
_aTotowa, NJ _bHumana Press _c2012 |
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| 300 |
_a1 recurso en línea (XI, 440 páginas) _b70 ilustraciones, 8 ilustraciones a color |
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| 336 |
_atexto _btxt _2rdacontent |
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| 337 |
_aelectrónico _bc _2rdamedia |
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| 338 |
_arecurso electrónico _bcr _2rdacarrier |
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| 347 |
_aarchivo de texto _bPDF |
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| 490 | 0 |
_aMethods in Molecular Biology _x1940-6029 _v867 |
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| 520 | _a"Next generation" sequencing techniques allow for more detailed analysis of exons and introns in multiple genes at the same time. This will reveal many mutations that potentially lead to exon skipping. To functionally test these a lot can be achieved with a limited set of protocols, while for the intentional induction of exon skipping different tools and target genes are involved and the translational path from in vitro splicing to in vivo tests in animal models requiring a more extensive set of protocols. Exon Skipping: Methods and Protocols provides scientist with a comprehensive guide to many of the methods and techniques used for exon skipping, such as methods on how to discriminate "real polymorphisms" from mutations that affect splicing. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical Exon Skipping: Methods and Protocols seeks to aid scientists in the continuing study of exon skipping. | ||
| 700 | 1 |
_aAartsma-Rus, Annemieke _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 776 | 0 | 8 |
_iPrinted edition: _z9781617797668 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781617797682 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781493962068 |
| 856 | 4 | 0 |
_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-767-5 _z(usuarios Universidad Europea de Valencia) |
| 942 |
_2lcc _cLE |
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| 988 | _aSpringer_Protocols_2012 | ||
| 999 |
_c234595 _d234595 |
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