000			04367nam a22003615i 4500
001			234596
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007			cr nn 008mamaa
008			110708s2011 xxu| s |||| 0|eng d
020			_a9781617791796
024	7		_a10.1007/978-1-61779-179-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMethods for the Discovery and Characterization of G Protein-Coupled Receptors _cedited by Craig W. Stevens.
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XIII, 428 páginas) _b81 ilustraciones, 5 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v60
505	0		_aIn Silico Identification of Novel G Protein-Coupled Receptors -- Alternative Pre-mRNA Splicing of G Protein-Coupled Receptors -- Detecting Polymorphisms in G Protein-Coupled Receptor Genes -- Regulation of the Transcription of G Protein-Coupled Receptor Genes -- Deciphering the Evolution of G Protein-Coupled Receptors in Vertebrates -- A New Approach to Visualize Endogenously Expressed G Protein-Coupled Receptors in Tissues and Living Cells -- Post-Translational Regulation of G Protein-Coupled Receptors -- Discovering G Protein-Coupled Receptor Motifs Mediating Export from the Endoplasmic Reticulum -- Identifying G Protein-Coupled Receptor Escorts, Chaperones, and Intracellular Tethers Regulating Receptor Density at the Cell Surface -- A Novel Method for Determining the Kinetics of G Protein-Coupled Receptor Plasma Membrane Expression -- Characterizing the Pharmacology of G Protein-Coupled Receptors in Transfected Cell Lines -- Novel Assay Technologies for the Discovery of G Protein-Coupled Receptor Drugs -- Discovering Cell-Type-Specific Patterns of G Protein-Coupled Receptor Phosphorylation -- Quantifying Allosteric Modulation of G Protein-Coupled Receptors -- Experimental Designs for the Study of Receptor-Receptor Interactions -- Elucidating Agonist-Selective Mechanisms of G Protein-Coupled Receptor Desensitization -- Detecting the Role of Arrestins in G-Protein Coupled Receptor Regulation -- Characterizing Molecular Mobility and Membrane Interactions of G Protein-Coupled Receptors -- Using RNA Interference to Downregulate G Protein-Coupled Receptors -- Upregulating G Protein-Coupled Receptors with Receptor Antagonists.
520			_aThe goal of the characterization and discovery of G protein-coupled receptors, arguably the most important class of signaling molecules in humans and other vertebrates, has spawned numerous vital methodologies.  In Methods for the Discovery and Characterization of G Protein-Coupled Receptors, experts in the field present the very latest on the methods and technology used to characterize and discover novel mechanisms of GPCRs which, in many cases, can be used directly to design experiments for the reader's particular GPCR of interest and their specific avenue of investigation. Divided into four convenient sections, this detailed volume covers GPCRs in the genome, trafficking of GPCRs, GPCRs on the membrane, as well as the regulation of these key receptors.  Chapters also feature an important section called "Future Directions" which gives the reader an insight into advances soon to be realized in each area. Written for the popular Neuromethods series, this book contains the kind of detailed description and implementation advice that is crucial for getting optimal results.   Authoritative and cutting-edge, Methods for the Discovery and Characterization of G Protein-Coupled Receptors serves as an ideal guide for scientists determined to further our knowledge of crucially important set of receptors.
700	1		_aStevens, Craig W _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617791789
776	0	8	_iPrinted edition: _z9781617791802
776	0	8	_iPrinted edition: _z9781493958511
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-179-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2011
999			_c234596 _d234596