000			03164nam a22003495i 4500
001			234601
003			ES-VaUE
005			20221220020613.0
007			cr nn 008mamaa
008			100301s1993 xxu| s |||| 0|eng d
020			_a9781592595068
024	7		_a10.1385/0896032361 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
100	1		_aGraham, John M. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
245	1	0	_aBiomembrane Protocols _bI. Isolation and Analysis _cby John M. Graham, Joan A. Higgins.
250			_a1st edition 1993
264		1	_aTotowa, NJ _bHumana Press _c1993
300			_a1 recurso en línea (X, 314 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v19
505	0		_aThe Identification of Subcellular Fractions from Mammalian Cells -- The Isolation of Nuclei and Nuclear Membranes from Rat Liver -- Isolation of Mitochondria, Mitochondrial Membranes, Lysosomes, Peroxisomes, and Golgi Membranes from Rat Liver -- Continuous-Flow Electrophoresis -- Fractionation of a Microsomal Fraction from Rat Liver or Cultured Cells -- Isolation of Plasma Membrane Sheets and Plasma Membrane Domains from Rat Liver -- The Analysis of Receptor-Mediated Endocytosis by Centrifugation in Isoosmotic Gradients -- The Isolation of Clathrin-Coated Vesicles and Purification of Their Protein Components -- Isolation of Membranes from Tissue Culture Cells -- The Isolation of Membranes from Bacteria -- Isolation and Purification of Functionally Intact Chloroplasts from Leaf Tissue and Leaf Tissue Protoplasts -- Isolation and Purification of Functionally Intact Mitochondria from Plant Cells -- Isolation of Cholinergic-Specific Synaptosomes by Immunoadsorption -- Separation and Analysis of Phospholipids by Thin Layer Chromatography -- Analysis of Phospholipids by High Performance Liquid Chromatography -- Measurement of Cholesterol in Membranes -- Analysis of Fatty Acids by Gas Liquid Chromatography -- Chemical Assays for Proteins -- Separation and Analysis of Membrane Proteins by SDS-Polyacrylamide Gel Electrophoresis -- Analysis of Membrane Proteins by Western Blotting and Enhanced Chemiluminescence -- Two-Dimensional Polyacrylamide Gel Electrophoresis of Membrane Proteins -- Isolation of a Membrane-Bound Enzyme, 5?-Nucleotidase -- The Isolation of Membrane Proteoglycans -- Oligosaccharide Mapping and Sequence Analysis of Glycosaminoglycans -- Analysis of Sugar Sequences in Glycoproteins by Glycosidase Digestion and Gel Filtration -- The Extraction and Analysis of Glycosphingolipids.
700	1		_aHiggins, Joan A. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
776	0	8	_iPrinted edition: _z9781627039253
776	0	8	_iPrinted edition: _z9780896032361
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032361 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1993
999			_c234601 _d234601