000			04591nam a22003735i 4500
001			234609
003			ES-VaUE
005			20221220020613.0
007			cr nn 008mamaa
008			100623s2010 xxu| s |||| 0|eng d
020			_a9781603273251
024	7		_a10.1007/978-1-60327-325-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRetinoids _bMethods and Protocols _cedited by Hui Sun, Gabriel H. Travis.
250			_a1st edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (X, 350 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v652
505	0		_aQuantification of Endogenous Retinoids -- Culture of Highly Differentiated Human Retinal Pigment Epithelium for Analysis of the Polarized Uptake, Processing, and Secretion of Retinoids -- Feeder-Independent Culture of Mouse Embryonic Stem Cells Using Vitamin A/Retinol -- In Vitro Assays of Rod and Cone Opsin Activity: Retinoid Analogs as Agonists and Inverse Agonists -- Physiological Studies of the Interaction Between Opsin and Chromophore in Rod and Cone Visual Pigments -- Measurement of the Mobility of All-Trans-Retinol with Two-Photon Fluorescence Recovery After Photobleaching -- Microfluorometric Measurement of the Formation of All-Trans-Retinol in the Outer Segments of Single Isolated Vertebrate Photoreceptors -- HPLC?/?MSN Analysis of Retinoids -- Binding of Retinoids to ABCA4, the Photoreceptor ABC Transporter Associated with Stargardt Macular Degeneration -- Fluorescence-Based Technique for Analyzing Retinoic Acid -- The Interaction Between Retinol-Binding Protein and Transthyretin Analyzed by Fluorescence Anisotropy -- Assay of Retinol-Binding Protein-Transthyretin Interaction and Techniques to Identify Competing Ligands -- Molecular Biology and Analytical Chemistry Methods Used to Probe the Retinoid Cycle -- Visualization of Retinoid Storage and Trafficking by Two-Photon Microscopy -- Reverse-Phase High-Performance Liquid Chromatography (HPLC) Analysis of Retinol and Retinyl Esters in Mouse Serum and Tissues -- Detection of Retinoic Acid Catabolism with Reporter Systems and by In Situ Hybridization for CYP26 Enzymes -- Diet in Vitamin A Research -- Experimental Approaches to the Study of A2E, a Bisretinoid Lipofuscin Chromophore of Retinal Pigment Epithelium -- Analysis of the Retinoid Isomerase Activities in the Retinal Pigment Epithelium and Retina -- Techniques to Study Specific Cell-Surface Receptor-Mediated Cellular Vitamin A Uptake.
520			_aNew and exciting biological functions are still being discovered for vitamin A derivatives, including the vast number of physiological activities of retinoids. In Retinoids: Methods and Protocols, expert researchers in the field present the most recent technical tools with diverse techniques for both in vitro and in vivo studies. Combining biochemical, biophysical, and cell biological techniques, the book addresses topics such as the detection and quantitation of retinoids using HPLC, mass spectrometry, and fluorescence, fluorescence anisotropy of retinol binding protein, cell culture models for studying retinoid transport and the role of retinol in embryonic stem cell culture, as well as many other detailed procedures. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinoids: Methods and Protocols seeks to aid beginning and experienced researchers from widely varied fields in the search to uncover even more vital aspects of vitamin A's impact on the human body.
700	1		_aSun, Hui _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aTravis, Gabriel H _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781603273244
776	0	8	_iPrinted edition: _z9781617796906
776	0	8	_iPrinted edition: _z9781607617679
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-325-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2010
999			_c234609 _d234609