000			04082nam a22003615i 4500
001			234613
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007			cr nn 008mamaa
008			151223s2016 xxu| s |||| 0|eng d
020			_a9781493933419
024	7		_a10.1007/978-1-4939-3341-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aProteomics in Systems Biology _bMethods and Protocols _cedited by Jörg Reinders.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XII, 313 páginas) _b76 ilustraciones, 61 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1394
505	0		_aMultiplexed Quantitative Proteomics for High-Throughput Comprehensive Proteome Comparisons of Human Cell Lines.- ample Preparation Approaches for iTRAQ Labelling and Quantitative Proteomic Analyses in Systems Biology.- Two birds with One Stone: Parallel Quantification of Proteome and Phospho-Proteome Using iTRAQ -- Selected Reaction Monitoring to Measure Proteins of Interest in Complex Samples: A Practical Guide.- Monitoring PPARG Induced Changes in Glycolysis by Selected Reaction Monitoring Mass Spectrometry.- A Targeted MRM Approach for Tempo-Spatial Proteomics Analyses.- Targeted Phospho-Proteome Analysis Using Selected/Multiple Reaction Monitoring (SRM/MRM) -- Testing Suitability of Cell Cultures for SILAC-Experiments Using SWATH-Mass Spectrometry.- Combining Amine-Reactive Cross-Linkers and Photo-Reactive Amino Acids for 3D-Structure Analysis of Proteins and Protein Complexes.- Tissue MALDI Mass Spectrometry Imaging (MALDI MSI) of Peptides -- Ethyl Esterification for MALDI-MS Analysis of Protein Glycosylation.- Characterization of Protein N-Glycosylation by Analysis of ZIC-HILIC-Enriched Intact Proteolytic Glycopeptides.- Simple and Effective Affinity Purification Procedures for Mass Spectrometry-Based Identification of Protein-Protein Interactions in Cell Signaling Pathways.- A Systems Approach to Understand Antigen Presentation and the Immune Response -- Profiling of Smal l Molecules by Chemical Proteomics.- Generating Sample Specific Databases for Mass Spectrometry Based Proteomic Analysis by Using RNA Sequencing.- A Proteomic Workflow Using High Throughput De Novo Sequencing towards Complementation of Genome Information for Improved Comparative Crop Science -- From Phospho-Proteome to Modelling of Plant Signalling Pathways.- Interpretation of Quantitative Shotgun Proteomic Data.- A Simple Workflow for Large Scale Shotgun Glycoproteomics.- Systemic Analysis of Regulated Functional Networks.  .
520			_aThis volume presents an overview of contemporary quantitative proteomics methods along with instructions on data interpretation, while providing examples on how to implement proteomics into systems biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Proteomics in Systems Biology: Methods and Protocols is a valuable resource for researchers who are interested in using proteomics techniques to help answer biological and medical questions.
700	1		_aReinders, Jörg _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493933396
776	0	8	_iPrinted edition: _z9781493933402
776	0	8	_iPrinted edition: _z9781493980253
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3341-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234613 _d234613