000			03622nam a22003735i 4500
001			234615
003			ES-VaUE
005			20221220020614.0
007			cr nn 008mamaa
008			100715s2002 xxu| s |||| 0|eng d
020			_a9781592592432
024	7		_a10.1385/1592592430 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHeme, Chlorophyll, and Bilins _bMethods and Protocols _cedited by Alison Smith, Michael Witty.
250			_a1st edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (IX, 340 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aLaboratory Methods for the Study of Tetrapyrroles -- Syntheses of Tetrapyrroles -- General Laboratory Methods for Tetrapyrroles -- Enzymatic Preparation of Tetrapyrrole Intermediates -- Analysis of Biosynthetic Intermediates, 5-Aminolevulinic Acid to Heme -- Analysis of Intermediates and End Products of the Chlorophyll Biosynthetic Pathway -- Analysis of Heme and Hemoproteins -- Hemoproteins Purification and Characterization by Using Aqueous Two-Phase Systems -- Structural Study of Heme Proteins by Electron Microscopy of 2-Dimensional Crystals -- Analysis and Reconstitution of Chlorophyll-Proteins -- Two-Dimensional Crystallization of Chlorophyll Proteins -- Biosynthesis and Analysis of Bilins -- Analysis and Reconstitution of Phytochromes -- Analysis and Reconstitution of Phycobiliproteins: Methods for the Characterization of Bilin Attachment Reactions.
520			_aAlthough researchers can profitably investigate heme, chlorophyll, and related tetrapyrroles in a wide range of academic and medical research programs, the handling and manipulation of these delicate compounds requires considerable skill and cross-boundary knowledge. In Heme, Chlorophyll, and Bilins: Methods and Protocols, an interdisciplinary panel of hands-on investigators overcomes these limitations by describing in detail how to work successfully with chlorophyll, heme, and bilins in biological, medical, chemical, and biochemical research. Each method is presented by a researcher who actually uses it on a daily basis and includes step-by-step instructions and pertinent tricks-of-the-trade that often make the difference between laboratory success and failure. Topics range from methods for the analysis of tetrapyrroles, heme, and hemoproteins, to the biosynthesis and the analysis of chlorophyll and bilins. Timely and highly practical, Heme, Chlorophyll, and Bilins: Methods and Protocols is a gold-standard collection of readily reproducible techniques suitable for a wide range of researchers, whether it be a clinician studying photodynamic therapy, an ecologist studying the chlorophyll composition of leaves in a tropical forest, or a cell biologist investigating the function of specific hemoproteins.
700	1		_aSmith, Alison _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWitty, Michael _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617373657
776	0	8	_iPrinted edition: _z9781588291110
776	0	8	_iPrinted edition: _z9781489938657
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592430 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2002
999			_c234615 _d234615