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007			cr nn 008mamaa
008			111117s2012 xxu| s |||| 0|eng d
020			_a9781617793790
024	7		_a10.1007/978-1-61779-379-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRibosome Display and Related Technologies _bMethods and Protocols _cedited by Julie A. Douthwaite, Ronald H. Jackson.
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XIII, 424 páginas) _b57 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v805
505	0		_aRibosome Display: A Perspective -- Preparation and Testing of E.coli S30 In Vitro Transcription Translation Extracts -- Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate -- Stabilized Ribosome Display for In Vitro Selection -- Eukaryotic Ribosome Display with In Situ DNA Recovery -- mRNA Display Using Covalent Coupling of mRNA to Translated Proteins -- SNAP Display: In Vitro Protein Evolution in Microdroplets -- cDNA Display: Rapid Stabilization of mRNA Display -- Optimization of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis -- Affinity Maturation of Phage Display Antibody Populations using Ribosome Display -- Evolution of Protein Stability Using Ribosome Display -- Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries -- Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display -- Peptide Screening Using PURE Ribosome Display -- Rapid Selection of High Affinity Binders Using Ribosome Display -- mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries -- Identification of Candidate Vaccine Genes Using Ribosome Display -- Ribosome Display for the Selection of Sac7d Scaffolds -- Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers -- Update on Pure Translation Display with Unnatural Amino Acid Incorporation -- In Vitro Selection of Unnatural Cyclic Peptide Libraries Via mRNA Display -- Optimization of CAT-354, a Therapeutic Antibody Directed Against Interleukin-13, Using Ribosome Display -- Affinity Maturation and Functional Dissection of a Humanized Anti-RAGE Monoclonal Antibody by Ribosome Display.
520			_aDisplay technologies have become a very powerful way of generating therapeutic lead molecules and specific reagents for increasing our understanding of biology; however, despite being first described shortly after phage display, the use of ribosome display and related methods have been much less widespread.  Since this is in part due to the complexity of the methods, Ribosome Display and Related Technologies: Methods and Protocols seeks to extend their use by collecting expert contributions describing these detailed protocols.  The protocols described range from well-established methods that have been used for a decade to generate high affinity antibodies, which are already in the clinic, to methods that are in their early stages of application such as display of peptides incorporating non-canonical amino acids.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Invaluable and easy to use, Ribosome Display and Related Technologies: Methods and Protocols will be of great benefit to those with general molecular biology or protein engineering experience who wish to select peptides or proteins by display, those with phage display experience who would benefit from the application of ribosome display, as well as those with some ribosome display experience who would like to expand the range of applications to which they are applying the technology.
700	1		_aDouthwaite, Julie A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aJackson, Ronald H _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617793783
776	0	8	_iPrinted edition: _z9781617793806
776	0	8	_iPrinted edition: _z9781493958726
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-379-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2012
999			_c234617 _d234617