000			03921nam a22003615i 4500
001			234625
003			ES-VaUE
005			20221220020614.0
007			cr nn 008mamaa
008			151109s2016 xxu| s |||| 0|eng d
020			_a9781493931972
024	7		_a10.1007/978-1-4939-3197-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNucleic Acid Aptamers _bSelection, Characterization, and Application _cedited by Günter Mayer.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XI, 242 páginas) _b49 ilustraciones, 29 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1380
505	0		_aSelection of Aptamers for Metabolite Sensing and Construction of Optical Nanosensors -- SELEX of Cell-Specific RNA Aptamers -- Developing Aptamers by Cell-Based SELEX -- DNA Aptamer Generation by Genetic Alphabet Expansion SELEX (ExSELEX) Using an Unnatural Base Pair System -- Capillary Electrophoresis for the Selection of DNA Aptamers Recognizing Activated Protein C -- Preparation of SELEX Samples for Next-Generation Sequencing -- Next Generation Analysis of Deep Sequencing Data: Bringing Light into the Black Box of SELEX Experiments -- Aptamer Binding Studies Using MicroScale Thermophoresis -- Label-Free Determination of the Dissociation Constant of Small Molecule-Aptamer Interaction by Isothermal Titration Calorimetry -- Applications of Aptamers in Flow and Imaging Cytometry -- In Vitro and In Vivo Imaging of Fluorescent Aptamers -- Crystallographic Pursuit of a Protein-RNA Aptamer Complex -- Voltammetric Aptasensor Based on Magnetic Beads Assay for Detection of Human Activated Protein C -- Apta-PCR -- Aptamer-Based Enzyme-Capture-Assay for Measurement of Plasma Thrombin Levels -- Application of Aptamers in Histopathology -- Aptamer Stainings for Super-Resolution Microscopy -- Synthesis and Characterization of Aptamer Targeted SNALPs for the Delivery of siRNA -- Screening of Genetic Switches Based on the Twister Ribozyme Motif.
520			_aThis volume provides protocol references covering recent developments in the aptamer field. Within the last decade, aptamers have become more and more popular, and their sophisticated biophysical properties together with their ability to be easily modified and, thus, adapted to various regimens makes them a very promising class of compounds. Divided into three sections, the book covers selection, a series of analytical methods to assess biophysical properties of aptamer-target interactions, as well as various applications of aptamers.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and easy to follow, Nucleic Acid Aptamers: Selection, Characterization, and Application provides a state-of-the-art summary of recent developments in the aptamer field and will be a helpful resource for scientists in the life sciences working with aptamers as tools to elucidate biological systems.
700	1		_aMayer, Günter _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493931965
776	0	8	_iPrinted edition: _z9781493931989
776	0	8	_iPrinted edition: _z9781493949342
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3197-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234625 _d234625