000			03666nam a22003615i 4500
001			234628
003			ES-VaUE
005			20221220020614.0
007			cr nn 008mamaa
008			140409s2014 xxu| s |||| 0|eng d
020			_a9781493904709
024	7		_a10.1007/978-1-4939-0470-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPhotoswitching Proteins _bMethods and Protocols _cedited by Sidney Cambridge.
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (X, 278 páginas) _b58 ilustraciones, 53 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1148
505	0		_aPhotoinduced Damage Resulting from Fluorescence Imaging of Live Cells -- Modification of Purified Proteins with Photochemical Protection Compounds for High-Resolution Photoactivation of Protein Function In Vitro and In Vivo -- Optochemical Activation of Kinase Function in Live Cells -- Photoswitching of Cell Surface Receptors Using Tethered Ligands -- Photocontrol of AMPA Receptors with a Photochromic Ligand -- Photoconversion of CFP to Study Neuronal Tissue with Electron Microscopy -- Light-Inducible Gene Regulation with Engineered Zinc Finger Proteins -- Manipulation of Plasma Membrane Phosphoinositides using Photoinduced Protein-protein Interactions -- A Roadmap to Applying Optogenetics in Neuroscience -- Salvaging Ruins: Reverting Blind Retinas into Functional Visual Sensors -- Photoactivated Adenylyl Cyclases as Optogenetic Modulators of Neuronal Activity -- Structural Basis of Photoswitching in Fluorescent Proteins -- Using Photoactivatable GFP to Track Axonal Transport Kinetics -- In Vivo Cell Tracking Using PhOTO Zebrafish -- In vivo Optogenetics for Light-induced Oxidative Stress in Transgenic Zebrafish  Expressing the KillerRed Photosensitizer Protein -- Photoactivatable Fluorescent Proteins for Super-Resolution Microscopy -- pcSOFI as a Smart Label-based Superresolution Microscopy Technique.
520			_aPhotoswitching Proteins: Methods and Protocols, focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of proteins, are now successfully used in almost all fields of biological research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Photoswitching Proteins: Methods and Protocols highlights many of the possibilities for research based on light-inducible, high-resolution manipulation of biological systems.
700	1		_aCambridge, Sidney _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493904716
776	0	8	_iPrinted edition: _z9781493904693
776	0	8	_iPrinted edition: _z9781493954353
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0470-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c234628 _d234628