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008			110923s2011 xxu| s |||| 0|eng d
020			_a9781617793196
024	7		_a10.1007/978-1-61779-319-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNanoproteomics _bMethods and Protocols _cedited by Steven A. Toms, Robert J. Weil.
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XIV, 322 páginas) _b68 ilustraciones, 34 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v790
505	0		_aMALDI-TOF Serum Profiling Using Semi-Automated Serum Peptide Capture with Magnetic Reversed Phase (C18) Beads -- Proteomics of Epithelial Lining Fluid Obtained by Bronchoscopic Microprobe Sampling -- Protein Identification Using Nano-HPLC-MS: ESI-MS and MALDI-MS Interfaces -- Three Dimensional Peptide Fractionation for Highly Sensitive Nanoscale LC-Based Shotgun Proteomic Analysis of Complex Protein Mixtures -- Nanospray Ion Mobility Mass Spectrometry of Selected High Mass Species -- Nanoelectrospray-MSn of Native and Permethylated Glycans -- N-linked Global Glycan Profiling by NanoLC Mass Spectrometry -- Applications of Nanoscale Liquid Chromatography Coupled to Tandem Mass Spectrometry in Quantitative Studies of Protein Expression, Protein-Protein Interaction, and Protein Phosphorylation -- Nano LC-MS/MS: A Robust Setup for Proteomic Analysis -- Nanofluidic Devices for Rapid Continuous-Flow Bioseparation -- Quantifying Attomole Amounts of Proteins from Complex Samples by Nano-LC and Selected Reaction Monitoring (SRM) -- A Sample Preparation Method for Gold Nanoparticle-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry -- Nanostructured TiO2 Thin Films for Phosphoproteomics Studies with MALDI Mass Spectrometry -- Nanofilament Silicon for Matrix-Free Laser Desorption/Ionization Mass Spectrometry -- Protein Nanoarrays for High Resolution Patterning of Bacteria on Gold Surfaces -- Engineered Multifunctional Nanotools for Biological Applications -- The Selective Capture of Phosphopeptides by Zirconium Phosphonate-Modified Magnetic Nanoparticles -- Nanowire Biosensors for Label-Free, Real-Time, Ultrasensitive Protein Detection -- Generation of Anti-Infectome/Anti-Proteome Nanobodies -- Isolation, Propagation, and Analysis of Biological Nanoparticles -- Functionalized Soluble Nanopolymers for Phosphoproteome Analysis -- Elucidating Structural Dynamics of Integral Membrane Proteins on Native Cell Surface by Hydroxyl Radical Footprinting and NanoLC-MS/MS -- Application of Electrostatic Repulsion Hydrophilic Interaction Chromatography (ERLIC) to the Characterization of Proteome, Glycoproteome, and Phosphoproteome Using Nano LC-MS/MS.
520			_aAs two relatively new fields of study, proteomics and nanotechnology have developed in parallel with each other to allow an increased precision in the identification of post-translational protein modifications as well as to provide a more automated isolation and detection of rare proteins in both serum and tissues.  The Nanoproteomics: Methods and Protocols volume organizes and collects technical advances from leaders in the field to make laboratory protocols more readily available and understandable to those who are attempting to incorporate nanotechnologic techniques into their proteomic research. Conveniently divided into five sections, this detailed volume covers preliminary sample preparation, nanoscale fluidic devices and methods, nanostructured surfaces and nanomaterials, and nanoproteomic techniques to detect and understand protein and proteomic alterations specific to human pathology. Written in the highly successful series entitled Methods in Molecular Biology™, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols that are readily reproducible, and tips on troubleshooting and avoiding known pitfalls.   Convenient and authoritative, Nanoproteomics: Methods and Protocols offers key procedures that are culled from the laboratories of leaders in the field of nanoproteomics with the aim of helping researchers in their standardization and proliferation of protocols that will lead to a more wide scale adoption and smoother progress in this vital field.
700	1		_aToms, Steven A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWeil, Robert J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617793189
776	0	8	_iPrinted edition: _z9781617793202
776	0	8	_iPrinted edition: _z9781493957996
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-319-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2011
999			_c234631 _d234631