000			04178nam a22003735i 4500
001			234640
003			ES-VaUE
005			20221220020615.0
007			cr nn 008mamaa
008			110909s2011 xxu| s |||| 0|eng d
020			_a9781617792823
024	7		_a10.1007/978-1-61779-282-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSingle Molecule Analysis _bMethods and Protocols _cedited by Erwin J. G. Peterman, Gijs J. L. Wuite.
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (X, 317 páginas) _b85 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v783
505	0		_aIntroduction to Optical Tweezers: Background, System Designs, and Commercial Solutions -- Optical Trapping and Unfolding of RNA -- DNA Unzipping and Force Measurements with a Dual Optical Trap -- Probing the Force Generation and Stepping Behavior of Cytoplasmic Dynein -- A Brief Introduction to Single-molecule Fluorescence Methods -- Fluorescent Labeling of Proteins -- Fluorescence Imaging of Single Kinesin Motors on Immobilized Microtubules -- Exploring Protein Superstructures and Dynamics in Live Bacterial Cells using Single-molecule and Superresolution Imaging -- Fluorescence Microscopy of Nanochannel-confined DNA -- Fluorescence Correlation Spectroscopy -- Introduction to Atomic Force Microscopy -- Sample Preparation for SFM Imaging of DNA, Proteins, and DNA-protein Complexes -- Single Molecule Protein Unfolding and Refolding using Atomic Force Microscopy -- How to Perform a Nanoindentation Experiment on a Virus -- Magnetic Tweezers for Single-molecule Manipulation -- Probing DNA topology using Tethered Particle Motion.
520			_aLife scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in Single Molecule Techniques: Methods and Protocols are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Single Molecule Techniques: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology.
700	1		_aPeterman, Erwin J. G _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWuite, Gijs J. L _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617792816
776	0	8	_iPrinted edition: _z9781617792830
776	0	8	_iPrinted edition: _z9781493958290
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-282-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2011
999			_c234640 _d234640