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020 _a9781607618959
024 7 _a10.1007/978-1-60761-895-9
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aEnzyme Stabilization and Immobilization
_bMethods and Protocols
_cedited by Shelley D. Minteer.
250 _a1st edition 2011
264 1 _aTotowa, NJ
_bHumana Press
_c2011
300 _a1 recurso en línea (X, 230 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v679
505 0 _aIntroduction to the field of enzyme immobilization and stabilization -- Stabilization of enzymes through encapsulation in liposomes -- Micellar enzymology for thermal, pH, and solvent stability -- Lipase activation and stabilization in room temperature ionic liquids -- Nanoporous silica glass for the immobilization of interactive enzyme systems -- Enzyme stabilization and immobilization by sol-gel entrapment -- Nanoporous Gold for Enzyme Immobilization -- Enzyme stabilization via bio-templated silicification reactions -- The immobilization of enzyme on Eupergit supports by covalent attachment -- Micellar polymer encapsulation of enzymes -- Enzyme Stabilization via Cross-Linked Enzyme Aggregates -- Enzyme immobilization in polyelectrolyte microcapsules -- Macroporous poly(GMA-co-EGDMA) for enzyme stabilization -- Enzyme-nanoparticle conjugates for biomedical applications -- Enzyme nanoparticle fabrication- Magnetic nanoparticle synthesis and enzyme immobilization -- Cytochrome C stabilization and immobilization in aerogels -- Kinetic Measurement for Enzyme Immobilization.
520 _aIn the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications. In Enzyme Stabilization and Immobilization: Methods and Protocols, expert researchers explore the latest developments through detailed laboratory protocols, which address many different theories and techniques in enzyme stabilization. Chapters outline protocols for enzyme stabilization in solutions, including: liposome formation, micelle introduction, crosslinking, and additives. Secondly, the book contain protocols for enzyme stabilization via enzyme immobilization, such as sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Enzyme Stabilization and Immobilization: Methods and Protocols is an essential handbook for all molecular biologists, biochemists, and biomedical and biochemical engineers.
700 1 _aMinteer, Shelley D
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781607618942
776 0 8 _iPrinted edition:
_z9781607618966
776 0 8 _iPrinted edition:
_z9781493957927
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-895-9
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2011
999 _c234649
_d234649