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008			140303s2014 xxu| s |||| 0|eng d
020			_a9781627039710
024	7		_a10.1007/978-1-62703-971-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPolyadenylation _bMethods and Protocols _cedited by Joanna Rorbach, Agnieszka J. Bobrowicz.
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (XIV, 377 páginas) _b63 ilustraciones, 22 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1125
505	0		_a3' end Formation and Regulation of Eukaryotic mRNAs -- Poly(A) Polymerase-based Poly(A) Length assay -- Using Klenow Mediated Extension to Measure Poly(A)-tail Length and Position in the Transcriptome -- Detection of Uridylated mRNAs -- Cytoplasmic Polyadenylation Assays -- In vitro Analysis of Transcriptional Activators and Polyadenylation -- Fluorescence Detection of Polyadenylation Reaction through the Coordination of Adensoine2-coralyne-adensoine2 -- Small molecule recognition of poly(A).-The Stem-loop Luciferase assay for Polyadenylation (SLAP) Method for Determining CstF-64-dependent Polyadenylation Activity -- Accurate Mapping of Cleavage and Polyadenylation Sites by 3' Region Extraction and Deep Sequencing -- Polyadenylation Site Prediction using PolyA-iEP Method -- Detecting alternative polyadenylation from Microarray Data -- Quantitative Polyadenylation Site Mapping with Single-molecule Direct RNA Sequencing -- RHAPA: A New Method to Quantify Alternative Polyadenylation -- Multiplex Analysis of PolyA-linked Sequences (MAPS): An RNA-seq Strategy to Profile poly(A+) RNA -- Global and Quantitative Profiling of Polyadenylated RNAs using PAS-seq -- High-throughput Sequencing of RNA Isolated by Crosslinking and Immunoprecipitation (HITS-CLIP) to Determine Sites of Binding of CstF-64 on Nascent RNAs -- Polyadenylation in Bacteria and Organelles -- In vivo Analysis of Polyadenylation in Prokaryotes -- Polyadenylation of RNA in E. coli: RNA Polymerase-associated (rA)n-synthetic Activities -- Analysis of the Human Mitochondrial Transcriptome using Directional Deep sequencing and Parallel Analysis of RNA Ends -- Measurement of Mitochondrial RNA Stability by Metabolic Labeling of Transcripts with 4-Thiouridine -- Deadenylation and its Regulation in Eukaryotic Cells -- Assaying mRNA Deadenylation in vitro -- Assaying mRNA Deadenylation in vivo -- In vitro Analysis of RNA Degradation Catalyzed by Deadenylase Enzymes -- microRNA-mediated Deadenylation in a Mammalian Cell-free System -- Quantitative Analysis of Deadenylation-independent mRNA Decay by a Modified MBRACE Assay.
520			_aIn Polyadenylation: Methods and Protocols, expert researchers in the field detail many of the protocols which are now commonly used to study polyadenylation.  Focusing on recent advances in the fast-moving polyadenylation filed, that has recently been recognized as a key contributor to the complexity of mammalian gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
700	1		_aRorbach, Joanna _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBobrowicz, Agnieszka J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627039727
776	0	8	_iPrinted edition: _z9781627039703
776	0	8	_iPrinted edition: _z9781493960552
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-971-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c234652 _d234652