000			03439nam a22003615i 4500
001			234655
003			ES-VaUE
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007			cr nn 008mamaa
008			160923s2017 xxu| s |||| 0|eng d
020			_a9781493940356
024	7		_a10.1007/978-1-4939-4035-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aEnhancer RNAs _bMethods and Protocols _cedited by Ulf Andersson Ørom.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 252 páginas) _b51 ilustraciones, 43 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1468
505	0		_aCellular Fractionation and Isolation of Chromatin-associated RNA -- Knockdown of Nuclear-located Enhancer RNAs and Long ncRNAs using Locked Nucleic Acid GapmeRs -- Visualization of Enhancer-derived Non-coding RNA -- UV-RNA Immunoprecipitation (UV-RIP) Protocol in Neurons -- Mapping Long non-coding RNA Chromatin Occupancy using Capture Hybridization Analysis of RNA Targets (CHART) -- Detecting long-range Enhancer-promoter Interactions by Quantitative Chromosome Conformation Capture -- Deciphering Noncoding RNA and Chromatin Interactions: Multiplex Chromatin Interaction Analysis by Paired-End Tag Sequencing (mChIA-PET) -- Identification of Transcribed Enhancers by Genome-wide Chromatin Immunoprecipitation Sequencing -- Global Run-On sequencing (GRO-seq) -- Computational Approaches for Mining GRO-seq Data to Identify and Characterize Active Enhancers -- Evaluating the Stability of mRNAs and Non-coding RNAs -- A Novel Method to Quantify RNA-protein Interactions in situ using FMTRIP and Proximity Ligation -- In silico Promoter Recognition from deepCAGE data -- Bioinformatics Pipeline for Transcriptome Sequencing Analysis -- CRISPR/Cas-9 Genome Editing in Embryonic Stem Cells -- Targeted Gene Activation Using RNA-Guided Nucleases.
520			_aThis volume provides a comprehensive overview of the experimental and computational methodologies used to study the function of long non-coding RNA (ncRNAs) expressed from enhancers. Chapter detail both wet-lab and dry-lab techniques and annotating long ncRNAs and exploring transcription by assessing where transcription starts and generally how it occurs.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Enhancer RNAs: Methods and Protocols aims to ensure successful results in this rapidly developing field.
700	1		_aØrom, Ulf Andersson _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493940332
776	0	8	_iPrinted edition: _z9781493940349
776	0	8	_iPrinted edition: _z9781493981601
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-4035-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234655 _d234655