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001			234656
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007			cr nn 008mamaa
008			141006s2015 xxu| s |||| 0|eng d
020			_a9781493915231
024	7		_a10.1007/978-1-4939-1523-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlasmodesmata _bMethods and Protocols _cedited by Manfred Heinlein.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (X, 346 páginas) _b53 ilustraciones, 39 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1217
505	0		_aPlasmodesmata: Channels for Intercellular Signaling During Plant Growth and Development -- Plasmodesmata: Channels for Viruses on the Move -- Imaging Plasmodesmata with High Resolution Scanning Electron Microscopy -- Preparative Methods for Imaging Plasmodesmata at Super-Resolution -- Isolation of Plasmodesmata from Arabidopsis Suspension Culture Cells -- Immunofluorescence Detection of Callose Deposition around Plasmodesmata Sites -- Imaging Callose at Plasmodesmata Using Aniline Blue: Quantitative Confocal Microscopy -- Localization of Fluorescently-Tagged Protein to Plasmodesmata by Correlative Light and Electron Microscopy -- Quantification of Plant Cell Coupling with Live-Cell Microscopy -- Drop-ANd-See: A Simple, Real-Time, and Non-Invasive Technique for Assaying Plasmodesmal Permeability -- Mapping Symplasmic Fields at the Shoot Apical Meristem Using Iontophoresis and Membrane Potential Measurements -- Analysis of the Conductivity of Plasmodesmata by Microinjection -- Investigating Plasmodesmata Genetics with Virus-Induced Gene Silencing and an Agrobacterium-Mediated GFP Movement Assay -- Probing Plasmodesmata Function with Biochemical Inhibitors -- GAL4 Transactivation-Based Assay for the Detection of Selective Intercellular Protein Movement -- Techniques for Assessing the Effects of Pharmacological Inhibitors on Intercellular Protein Movement -- Probing Protein Targeting to Plasmodesmata Using Fluorescence Recovery after Photo-Bleaching -- The Tracking of Intercellular Small RNA Movement -- Analysis of the Role of Myosins in Targeting Proteins to Plasmodesmata -- Pumilio-Based RNA In Vivo Imaging -- In Vivo RNA Labeling Using MS2.
520			_aPlasmodesmata: Methods and Protocols provides a unique compendium of currently applied methods to explore plasmodesmata (PD). Opening with overviews of PD structure and their function during development as well as the role of viruses as models for addressing cellular mechanisms in macromolecular trafficking and movement through PD, the book continues with detailed experimental protocols for imaging PD, the isolation and structural analysis of PD, the analysis of PD conductivity and regulation, and for studying cellular mechanisms by which macromolecules are targeted to PD and transported through the channel. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and easy to use, Plasmodesmata: Methods and Protocols delivers detailed information about specific practical procedures in order to enable their reproduction and application in PD-related research in laboratories around the world.
700	1		_aHeinlein, Manfred _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493915248
776	0	8	_iPrinted edition: _z9781493915224
776	0	8	_iPrinted edition: _z9781493946433
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1523-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c234656 _d234656