000			03419nam a22003615i 4500
001			234662
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007			cr nn 008mamaa
008			170825s2017 xxu| s |||| 0|eng d
020			_a9781493972838
024	7		_a10.1007/978-1-4939-7283-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSkeletal Muscle Development _cedited by James G. Ryall.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XII, 226 páginas) _b53 ilustraciones, 46 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1668
505	0		_aBisulfite Sequencing for DNA Methylation Analysis of Primary Muscle Stem Cells -- Whole Genome Chromatin IP-Sequencing (ChIP-Seq) in Skeletal Muscle Cells -- Analysis of RNA Expression in Adult Zebrafish Skeletal Muscle -- Chick Somite Electroporation for Gene Function Studies -- Targeted Lipidomic Analysis of Myoblasts by GC-MS and LC-MS/MS -- Measuring Mitochondrial Substrate Utilization in Skeletal Muscle Stem Cells -- Microcontact-Printed Hydrogel Microwell Arrays for Clonal Muscle Stem Cell Cultures -- Isolation, Culture, and Differentiation of Fibro/Adipogenic Progenitors (FAPs) from Skeletal Muscle -- Human Satellite Cell Isolation and Xenotransplantation -- Application of Split-GFP Reassembly Assay to Study Myogenesis and Myofusion In Vitro -- Myogenic Maturation by Optical-Training in Cultured Skeletal Muscle Cells -- Fabrication of Micromolded Gelatin Hydrogels for Long-Term Culture of Aligned Skeletal Myotubes -- Quantification of Embryonic Myofiber Development by Immunofluorescence -- How to Wire the Diaphragm: Wholemount Staining Methods to Analyze Mammalian Respiratory Innervation -- Membrane Repair Assay for Human Skeletal Muscle Cells -- Cryoinjury Model for Tissue Injury and Repair in Bioengineered Human Striated Muscle.
520			_aThis detailed volume presents a wide range of techniques that go beyond the standard assays typically used to assess Myogenesis. The content included addresses assays to analyze skeletal muscle gene expression, proliferating muscle cells, the process of myoblast fusion, muscle development (in vivo), as well as muscle repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Skeletal Muscle Development serves as an ideal guide to the study of this highly complex yet carefully regulated process.>.
700	1		_aRyall, James G _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493972821
776	0	8	_iPrinted edition: _z9781493972845
776	0	8	_iPrinted edition: _z9781493984411
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7283-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234662 _d234662