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001			234663
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008			170429s2017 xxu| s |||| 0|eng d
020			_a9781493969883
024	7		_a10.1007/978-1-4939-6988-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aZygotic Genome Activation _bMethods and Protocols _cedited by Kiho Lee.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 272 páginas) _b51 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1605
505	0		_aClearance of Maternal RNAs: Not a Mummy's Embryo Anymore -- Link of Zygotic Genome Activation and Cell Cycle Control -- Role of MicroRNAs in Zygotic Genome Activation: Modulation of mRNA during Embryogenesis -- Gene Expression Analysis of Mammalian Oocytes and Embryos by Quantitative Real-Time RT-PCR -- Detection of miRNA in Mammalian Oocytes and Embryos -- Detection of Bidirectional Promoter-Derived IncRNAs from Small-Scale Samples using Pre-Amplification-Free Directional RNA-Seq Method -- Detection and Characterization of Small Non-Coding RNAs in Mouse Gametes and Embryos Prior to Zygotic Genome Activation -- Purification of Zygotically Transcribed RNA through Metabolic Labeling of Early Zebrafish Embryos -- RNA FISH to Study Zygotic Genome Activation in Early Mouse Embryos -- Detection of RNA Polymerase II in Mice Embryos during Zygotic Genome Activation using Immunocytochemistry -- Immunological Staining of Global Changes in DNA Methylation in the Early Mammalian Embryo -- Single Cell Restriction Enzyme-Based Analysis of Methylation at Genomic Imprinted Regions in Preimplantation Mouse Embryos -- Use of Chemicals to Inhibit DNA Replication, Transcription, and Protein Synthesis to Study Zygotic Genome Activation -- Targeted Gene Knockdown in Early Embryos using siRNA -- Generating Mouse Models using Zygote Electroporation of Nucleases (ZEN) Technology with High Efficiency and Throughput -- CRISPR/Cas9-Mediated Gene Targeting during Embryogenesis in Swine -- Potential Involvement of SCF-Complex in Zygotic Genome Activation during Early Bovine Embryo Development -- Use of Histone K-M Mutants for the Analysis of Transcriptional Regulation in Mouse Zygotes.
520			_aThis volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammalian oocytes and embryos, RNA FISH to study ZGA in early mouse embryos, and CRISPR/Cas9-mediated gene targeting during embryogenesis in the mouse and pig. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Zygotic Genome Activation: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about practical approaches to monitor the changes seen during ZGA.
700	1		_aLee, Kiho _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493969869
776	0	8	_iPrinted edition: _z9781493969876
776	0	8	_iPrinted edition: _z9781493983599
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6988-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234663 _d234663