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008			170202s2017 xxu| s |||| 0|eng d
020			_a9781493967889
024	7		_a10.1007/978-1-4939-6788-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHistochemistry of Single Molecules _bMethods and Protocols _cedited by Carlo Pellicciari, Marco Biggiogera.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XIV, 341 páginas) _b70 ilustraciones, 59 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1560
505	0		_aSingle Cell Cytochemistry Illustrated by the Demonstration of Glucose-6-Phosphate Dehydrogenase Deficiency in Erythrocytes -- Autofluorescence Spectroscopy for Monitoring Metabolism in Animal Cells and Tissues -- Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree -- Histochemical Demonstration of Tripeptidyl Aminopeptidase I -- Enzyme Histochemistry for Functional Histology in Invertebrates -- Lectin Histochemistry: Historical Perspectives, State of the Art, and the Future -- Isolation of Viable Glycosylation-Specific Cell Populations for Further In Vitro or In Vivo Analysis Using Lectin-Coated Magnetic Beads -- Lectin Histochemistry for Metastasizing and Non-Metastasizing Cancer Cells -- The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium -- Heat-Induced Antigen Retrieval in Immunohistochemistry: Mechanisms and Applications -- Detecting Neuronal Differentiation Markers in Newborn Cells of the Adult Brain -- Characterizing Satellite Cells and Myogenic Progenitors During Skeletal Muscle Regeneration -- Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin Fixed and Paraffin Embedded Tissue -- Tissue Fixation and Processing for the Histological Identification of Lipids -- Staining Methods for Normal and Regenerative Myelin in the Nervous System -- Nile Red Staining of Neutral Lipids in Yeast -- Staining of Lipid Droplets with Monodansylpentane -- Fluorochromes for DNA Staining and Quantitation -- Osmium Ammine for Staining DNA at Electron Microscopy -- DNA Labeling at Electron Microscopy -- Visualizing RNA at Electron Microscopy by Terbium Citrate -- Two-Tailed Comet Assay (2T-Comet): Simultaneous Detection of DNA Single and Double Strand Breaks -- Detection of Endogenous Nuclear Proteins in Plant Cells: Localizing Nuclear Matrix Constituent Proteins (NMCPs), the Plant Analogues of Lamins -- Histochemical Analysis of Plant Secretory Structures -- A Histochemical Technique for the Detection of Annonaceous Acetogenins.
520			_aThis detailed volume explores numerous histochemical techniques through a series of lab-tested protocols for the detection of specific molecules or metabolic processes, both at light and electron microscopy. More in detail, the book is divided into six sections covering a variety of chemical targets. It begins with a section on vital histochemistry and continues with chapters on histochemistry as it relates to lectins, proteins, lipids, DNA and RNA, as well as plants. The volume also contains four overview chapters on vital histochemistry, lectin histochemistry, and DNA fluorochromes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Histochemistry of Single Molecules: Methods and Protocols aims to effectively help scientists in very different research fields to elucidate biological issues though a unique approach to molecular biology in situ.
700	1		_aPellicciari, Carlo _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBiggiogera, Marco _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493967872
776	0	8	_iPrinted edition: _z9781493967896
776	0	8	_iPrinted edition: _z9781493983018
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6788-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234664 _d234664