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020 _a9781592595952
024 7 _a10.1385/0896034771
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aTherapeutic Applications of Ribozymes
_cedited by Kevin J. Scanlon.
250 _a1st edition 1998
264 1 _aTotowa, NJ
_bHumana Press
_c1998
300 _a1 recurso en línea (XIV, 462 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Medicine
_x1940-6037
_v11
505 0 _aSmall Efficient Hammerhead Ribozymes -- The Hairpin Ribozyme -- Molecular Manipulations of the Catalytic RNAs from the Human Hepatitis Delta Virus -- The Design, Production, and Validation of an Anti-HIV Type 1 Ribozyme -- Methods for Treating HIV by Gene Therapy Using Ribozymes -- An Expression Vector for Multiple Ribozymes -- Viral RNA Targets for Ribozymes -- Hammerhead Ribozyme-Mediated Cleavage of Hepatitis B Virus RNA -- Bovine Leukemia Virus Ribozymes -- Use of Ribozymes to Specifically Alter the MDR-1 Phenotype -- Adenoviral-Mediated Anti-H-ras Ribozyme for the Treatment of Bladder Cancer -- Tissue-Specific Delivery of an anti-H-ras Ribozyme Against Malignant Melanoma -- Ribozymes as a Novel Approach for the Treatment of Human Pancreatic carcinoma -- K-rasRibozyme for Lung Cancer -- Anti-c-erb-B-2 Ribozyme for Breast Cancer -- Ribozymes -- Preformed Ribozymes -- Folate-Polylysine-Mediated Delivery of a Multiunit Anti-BCR/ABL Ribozyme to BCR/ABL-Transformed 32D Cells -- Ribozymes Targeted Against AML1/MTG8 -- Ribozyme Targeting of the Growth Factor Pleiotrophin -- Ribozyme-Mediated Inhibition of Cell Proliferation -- Inhibition of ?APP Synthesis by Ribozymes and Oligonucleotides in a Coupled Reticulocyte Transcription Translation System -- Selective Modification of Fatty Acid Synthesis Pathway by Expression of an Acetyl-CoA Carboxylase-Specific Ribozyme Gene -- In Vivo Knockout of a Tissue-Specific Gene by Synthetic Ribozymes -- Ribozyme-Mediated Downregulation of Gene Expression in Transgenic Mice -- Targeted Cleavage of RNA Using External Guide Sequences and Eukaryotic RNase P -- Application of In Vitro Selection in Design of External Guide Sequences That Direct Efficient Cleavage of RNA by RNase P -- A PCR-Based Approach to Hammerhead Ribozyme Synthesis -- Measurement of Ribozyme Activity Using RNase Protection.
520 _aIn Therapeutic Applications of Ribozymes, expert laboratory scientists describe in detail their methodologies for constructing ribozymes designed to elucidate the role of specific genes as key routes to the development of novel therapies for a wide variety of diseases. The authors review the many sites targeted with ribozymes in various diseases and provide specific accounts of the practical techniques required for the proper use of ribozymes in these systems. Their cutting-edge protocols demonstrate how to achieve ribozyme expression in distinct cellular systems, the preparation and use of high-efficiency ribozyme DNA or RNA delivery, and the studies required to prove the efficacy of ribozyme-mediated inhibition, with its concomitant effect on phenotypic parameters. Therapeutic Applications of Ribozymes contains all the practical detail necessary to realize the high promise of ribozyme technology as a significant, widely used methodology. Focused on ribozyme targets and the novel associated technology, the book immediately becomes the leading resource for all those seeking to transform this new category of therapeutic agent into efficacious clinical practice.
700 1 _aScanlon, Kevin J
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781489943439
776 0 8 _iPrinted edition:
_z9780896034778
776 0 8 _iPrinted edition:
_z9781489943422
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896034771
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_1998
999 _c234666
_d234666