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008			140326s2014 xxu| s |||| 0|eng d
020			_a9781493904280
024	7		_a10.1007/978-1-4939-0428-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHerpes Simplex Virus _bMethods and Protocols _cedited by Russell J. Diefenbach, Cornel Fraefel.
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XI, 331 páginas) _b45 ilustraciones, 28 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1144
505	0		_aHSV-1 Biology and Life Cycle -- Herpes Simplex Virus Growth, Preparation, and Assay -- Isolation of Herpes Simplex Virus Nucleocapsid DNA -- Construction and Characterization of Bacterial Artificial Chromosomes (BACs) Containing Herpes Simplex Virus Full-Length Genomes -- Engineering HSV-1 Vectors for Gene Therapy -- Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors -- HSV-1 Amplicon Vectors as Genetic Vaccines -- Modification of HSV-1 to an Oncolytic Virus -- Herpes Simplex Virus Mutant Generation and Dual Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo -- Phenotypic and Genotypic Testing of HSV-1 Resistance to Antivirals -- Using Homogeneous Primary Neuron Cultures to Study Fundamental Aspects of HSV-1 Latency and Reactivation -- Characterization of Extracellular HSV-1 Virions by Proteomics -- A Precipitation-Based Assay to Analyze Interactions of Viral Particles with Cytosolic Host Factors -- Affinity Purification Combined with Mass Spectrometry to Identify Herpes Simplex Virus Protein-Protein Interactions -- Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy -- Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication -- Expression, Purification and Crystallization of HSV-1 Glycoproteins for Structure Determination -- Cryo-EM Techniques to Resolve the Structure of HSV-1 Capsid-Associated Components -- In Vivo HSV-1 DNA Transport Studies Using Murine Retinal Ganglion Cells -- HSV-1 Protein Expression Using Recombinant Baculoviruses -- The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines.
520			_aHerpes Simplex Virus: Methods and Protocols provides a wide collection of protocols employed in various levels of herpes virus research, including basic protocols on growing viruses in cell culture and cloning, manipulating, and preparing viral DNA. Other chapters describe approaches to design and apply HSV-1 vectors for vaccination, cancer and gene therapy, or to study specific aspects of HSV-1 biology such as latency, intracellular transport, and protein-protein interaction. Procedures for structural analyses, microscopy, proteomics, and testing of antivirals are included as well.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and authoritative, Herpes Simplex Virus: Methods and Protocols will aid new researchers in the field of herpes virology as well as those experienced investigators wishing to embark on new techniques.
700	1		_aDiefenbach, Russell J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aFraefel, Cornel _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493904297
776	0	8	_iPrinted edition: _z9781493904273
776	0	8	_iPrinted edition: _z9781493954070
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0428-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c234673 _d234673