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020 _a9781493965816
024 7 _a10.1007/978-1-4939-6581-6
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPhagocytosis and Phagosomes
_bMethods and Protocols
_cedited by Roberto Botelho.
250 _a1st edition 2017
264 1 _aNew York, NY
_bSpringer International Publishing
_c2017
300 _a1 recurso en línea (XII, 360 páginas)
_b57 ilustraciones, 24 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1519
505 0 _aPhagocytosis: Hungry, Hungry Cells -- Analysis of Human and Mouse Neutrophil Phagocytosis by Flow Cytometry -- Quantitative Efferocytosis Assays -- Quantifying Phagocytosis by Immunofluorescence and Microscopy -- Single Cell Analysis of Phagocytosis, Phagosome Maturation, Phagolysosomal Leakage and Cell Death Following Exposure of Macrophages to Silica Particles -- Quantitative Live-cell Fluorescence Microscopy during Phagocytosis -- Intracellular Manipulation of Phagosomal Transport and Maturation Using Magnetic Tweezers -- Quantitative Immunofluorescence to Study Phagosome Maturation -- Using Fluorescence Resonance Energy Transfer-Based Biosensors to Probe Rho Gtpase Activation during Phagocytosis -- Analysis of LC3-associated Phagocytosis and Antigen Presentation -- Quantitative Spatio-Temporal Analysis of Phagosome Maturation in Live Cells -- Measuring Phagosomal pH by Fluorescence Microscopy -- Image-Based Analysis of Phagocytosis - Measuring Engulfment and Internalization -- Fluorometric Approaches to Measuring Reductive and Oxidative Events in Phagosomes -- Simultaneous Analysis of Multiple Lumenal Parameters of Individual Phagosomes Using High-Content Imaging -- <isolation and="" western="" blotting="" of="" latex-bead="" phagosomes="" to="" track="" phagosome="" maturation.-="" assessing="" the="" proteome="" by="" quantitative="" mass="" spectrometry.-="" dissecting="" phagocytic="" removal="" apoptotic="" cells="" in="" Caenorhabditis Elegans -- Measurement of Salmonella Enterica Internalization and Vacuole Lysis in Epithelial Cells -- Bacterial Binding, Phagocytosis and Killing: Measurements Using Colony Forming Units -- Filamentous Bacteria as Targets to Study Phagocytosis -- Growing and Handling of Mycobacterium Tuberculosis for Macrophage Infection Assays -- Mycobacterium Tuberculosis: Readouts of Bacterial Fitness and The Environment within the Phagosome -- Using Flow Cytometry to Analyse Cryptococcus Infection in Macrophages.>.
520 _aThis volume details experimental approaches used to investigate phagocytosis and phagosome maturation. Chapters present methods and protocols on quantifying uptake and phagosome maturation using biophysical and biochemical approaches, proteomics, microscopy, and flow cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phagocytosis and Phagosomes: Methods and Protocols aims to be an important resource for both experts in the field and for those investigators delving into phagocytosis and phagosome maturation for the first time.
700 1 _aBotelho, Roberto
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493965793
776 0 8 _iPrinted edition:
_z9781493965809
776 0 8 _iPrinted edition:
_z9781493982417
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6581-6
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2017
999 _c234693
_d234693