000			03022nam a22003375i 4500
001			234696
003			ES-VaUE
005			20221220020619.0
007			cr nn 008mamaa
008			100301s1995 xxu| s |||| 0|eng d
020			_a9781592595389
024	7		_a10.1385/0896032752 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aAntibody Engineering Protocols _cedited by Sudhir Paul.
250			_a1st edition 1995
264		1	_aTotowa, NJ _bHumana Press _c1995
300			_a1 recurso en línea (XII, 450 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v51
505	0		_aSEQHUNT -- Molecular Modeling of Antibody-Combining Sites -- Structure and Properties of Human Immunoglobulin Light-Chain Dimers -- Crystallographic and Chromatographic Methods for Study of Antibody Light Chains and Other Proteins -- Detection of Human Variable Gene Family Expression at the Single-Cell Level -- Purification of Reduced and Alkylated Antibody Subunits -- Murine Monoclonal Antibody Development -- Comparative Properties of Polyclonal and Monoclonal Antibodies -- Evaluation of Antibody Clonality -- Affinity Immunoblotting -- Epitope and Idiotope Mapping Using Monoclonal Antibodies -- Anti-Idiotypic Antibodies That Mimic Opioids -- Catalytic Antibodies -- Preparation and Assay of Acetylcholinesterase Antibody -- DNA Hydrolysis by Antibodies -- Screening Strategies for Catalytic Antitransition-State Analog Antibodies -- Expression of Chimeric Immunoglobulin Genes in Mammalian Cells -- Single-Chain Anti-DNA Fv -- Molecular Cloning of Antiground-State Proteolytic Antibody Fragments -- Cloning and Bacterial Expression of an Esterolytic sFv -- Site-Directed Mutagenesis of Antibody-Variable Regions -- Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation -- Chaperonins in Phage Display of Antibody Fragments -- Phage-Display Libraries of Murine and Human Antibody Fab Fragments -- Selection of Human Immunoglobulin Light Chains from a Phage-Display Library -- Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography -- Rapid Purification of Recombinant Antibody Fragments for Catalysis Screening -- Assay of Radiolabeled VIP Binding and Hydrolysis by Antibodies -- Methods of Measuring Thyroglobulin and Peptide-Methylcoumarinamide Hydrolysis by Autoantibodies -- Radiolabeling of Antibodies for Therapy and Diagnosis.
700	1		_aPaul, Sudhir _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489940742
776	0	8	_iPrinted edition: _z9780896032750
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032752 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1995
999			_c234696 _d234696