000			04475nam a22003975i 4500
001			234702
003			ES-VaUE
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007			cr nn 008mamaa
008			100301s1998 xxu| s |||| 0|eng d
020			_a9781592595709
024	7		_a10.1385/0896034941 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRNA Isolation and Characterization Protocols _cedited by Ralph Rapley, David L. Manning.
250			_a1st edition 1998
264		1	_aTotowa, NJ _bHumana Press _c1998
300			_a1 recurso en línea (XIII, 264 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v86
505	0		_ato Isolating RNA -- Large and Small Scale RNA Preparations from Eukaryotic Cells -- An Improved Rapid Method of Isolating RNA from Cultured Cells -- Isolating RNA with the Cationic Surfactant, Catrimox-14 -- RNA Extraction from Formalin-Fixed and Paraffin-Embedded Tissues -- Extraction and Purification of RNA from Plant Tissue Enriched in Polysaccharides -- Isolation of Plant Mitochondrial RNA from Green Leaves -- Extraction of RNA from Fresh and Frozen Blood -- Isolation of Total RNA from Bacteria -- Isolation of Total RNA from Tissues or Cell Lines -- Isolation of Messenger RNA -- UV Spectrophotometric Analysis of Ribonucleic Acids -- Formaldehyde Gel Electrophoresis of Total RNA -- Preparation of RNA Dot-Blots -- Nonradioactive Northern Blotting -- The Use of RNA Probes for the Analysis of Gene Expression -- Analysis of RNA by Northern Blotting Using Riboprobes -- RNA Quantitative Analysis from Fixed and Paraffin-Embedded Tissues -- Quantitative Analysis of RNA Species by PCR and Solid-Phase Minisequencing -- Preparation of Tissue Sections and Slides for mRNA Hybridization -- Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes -- One-Tube RT-PCR with Sequence-Specific Primers -- Identification of Differentially Expressed Genes by Nonradioactive Differential Display of Messenger RNA -- Characterization of RNA Using Continuous RT-PCR Coupled with ELOSA -- Gene Expression Analysis by CD-RT-PCR Eric de Kant -- Primer Extension Analysis of mRNA -- S1 Mapping Using Single-Stranded DNA Probes -- Measurements of Rate of Transcription in Isolated Nuclie by Nuclear "Run-Off" Assay -- Transcription In Vitro Using Bacteriophage RNA Polymerases -- In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System -- In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System -- The Xenopus Egg Extract Translation System -- Purification and Characterization of Viral dsRNA Genome Profiles by Crosshybridization.
520			_aRNA Isolation and Characterization Protocols offers biological investigators a powerful and highly useful collection of up-to-date methods for the isolation of RNA from a variety of sources, including bacterial, plant, and mammalian cells. These fine-tuned protocols take both the experienced and inexperienced investigator through the nuances of RNA manipulation, from extraction to in vitro translation. The authoritative researchers writing here also include detailed protocols for the analysis and characterization of isolated RNA species and extensive troubleshooting hints and tips to ensure easy reproducibility. RNA Isolation and Characterization Protocols gives today's bench scientists the single best collection of easily reproducible, step-by-step RNA techniques now available. This book will quickly become indispensable for everyone seeking to manipulate RNA and help set the stage for the coming revolution in RNA-based diagnostic techniques.
700	1		_aRapley, Ralph _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aManning, David L _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489942500
776	0	8	_iPrinted edition: _z9781627039512
776	0	8	_iPrinted edition: _z9780896034945
776	0	8	_iPrinted edition: _z9780896033931
776	0	8	_iPrinted edition: _z9781489942494
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896034941 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1998
999			_c234702 _d234702